Microsatellite markers for plants of the genus triticeae and use thereof

ABSTRACT

The invention relates to novel microsatellite markers for wheats ( Triticum aestivum ) and closely related species (Genus Triticeae) and to the use of said markers for the genetic analysis of plants of the  Triticum aestivum  species.

[0001] The invention relates to novel genetic, markers for wheats(Triticum aestivum L.) and closely related species (Tribus Triticeae)and to the use of said markers.

[0002] The most widely spread, known, DNA-based genetic markers are theso-called restriction, fragment length polymorphisms (RFLP) markers.,For using these markers, genomic DNA is digested with restrictionenzymes, separated on agarose gels and transferred to nylon membranes(Southern Blot). Specific fragments are detected by hybridization withradioactively labeled DNA probes. When mutations occur in the region ofthe restriction enzymes used or when smaller deletions/insertions occur,polymorphisms between different lines are found, which are passed onstably and mostly codominantly. The use of RFLP markers in hexaploidcultivated wheat is possible only to a limited extent, since only verylittle polymorphism is detected in wheat in this manner.

[0003] It has already been described that microsatellite markers detectsignificantly more polymorphism between different wheat lines than doRFLP markers. This can be attributed particularly to the occurrence ofmultiple alleles per locus (Röder et al., Mol. Gen. Genet. (1995) 246,327-333). Moreover, it is known that microsatellite markers have theadvantage that they can be detected by way of PCR and that thereforelarge amounts of samples can be analyzed more easily.

[0004] It is an object of the invention to provide novel microsatellitemarkers for the genetic analysis of plants of the Triticum aestivumspecies, which markers are distinguished by a degree of DNApolymorphism, which is higher than that of other molecular probes, thathave been developed previously for the wheat genome.

[0005] This objective is accomplished by claims 1 to 10. The inventivemarkers are based on the amplification of certain hypervariable genomesections, the so-called microsatellites, with the help of theirpolymerase chain reaction (PCR). For specific amplification, twoprimers, in each to the case left and the right in the flankingsequences, are required for each microsatellite locus. On the average,these primers are 20±3 bases long and are defined by their sequences. Inprinciple, a microsatellite marker is a sequence tagged site (STS),which is defined by two specific primers. These primers flank, in eachcase to the left and the right, a so-called microsatellite sequence. Amicrosatellite sequence is defined as a tandem repetitive repetition ofa di-, tri- or tetranucleotide sequence, for example (GA)_(n), in whichn≧10. Composite microsatellite sequences also occur, such as(GT)_(n)(AT)_(n), as well as imperfect sequences, in which individualbases are mutated, such as (GA)_(n)CA(GA)_(n). Among various lines andvarieties, there is variation in the number of repeats at a certainlocus. After amplification of the microsatellites, this leads, by meansof the specific primers in the flanking sequences, to PCR products ofdifferent length and, with that, to polymorphisms. These polymorphismsare passed on stably and can therefore be used as genetic markers. Insome cases, null alleles (no visible fragment) also occur, when thereare mutations within the binding site for the primers.

[0006] The separation and detection of the PCR products obtained can becarried out with different technical variants. For separating thefragments, highly resolving agarose gels, native polyacrylamide gels ordenaturing polyacrylamide gels (=sequencing gels) can be used. Dependingon the separation system, fragments are detected using ethidium bromidestaining, silver staining or, after labeling the PCR fragmentsradioactively, using autoradiography. A further, very effectivevariation for separation and detection consists of the use of anautomatic sequencer with dye- or fluorescence-labeled primers. For thispurpose, it is necessary to synthesize a dye- or fluorescence-labeledprimer from each microsatellite primer pair. PCR amplification resultsin a labeled product, which can be detected by the sequencing equipment.At the same time, dye- or fluorescence-labeled size standards are alsoseparated for each sample in the same track. After that, specialsoftware enable the absolute size of each fragment, which has beenseparated, to be calculated and, with that, also permits fragments fromdifferent gel runs to be compared. With this method, several hundredsamples can be analyzed largely automatically in a day.

[0007] Pursuant to the invention, microsatellite markers are madeavailable, which contain the following primer pairs with assignedmicrosatellite sequences or a number thereof and amplify the loci of allchromosomes of the wheat genome and therefore find use for gene marking.WMS Length (bp Annealing Number WMS Primer Left WMS Primer Right in “cs”Repeat Type Temperature WMS052 5′ CTA TGA GGC GGA GGT TGA AG 3′ (SEQ. IDNO. 1) 5′ TGC GGT GCT CTT CCA TTT 3′ (SEQ. ID NO. 2) 150 GTimp 60° C.WMS055 5′ GCA TCT GGT ACA CTA GCT GCC 3′ (SEQ. ID NO. 3) 5′ TCA TGG ATGCAT CAC ATC CT 3′ (SEQ. ID NO. 4) 127 CTimp 60° C. WMS057 5′ TCG ATT CTGAAA GGT TCA TCG 3′ (SEQ. ID NO. 5) 5′ CGA TCA AGT AGT TGA AAG CGC 3′(SEQ. ID NO. 6) 224 AAAAAimp 60° C. WMS058 5′ TCT GAT CCC GTG AGT GTAACA 3′ (SEQ. ID NO. 7) 5′ GAA AAA AAT TGC ATA TGA GCC C 3′ (SEQ. ID NO.8) 118 CA 60° C. WMS060 5′ TGT CCT ACA CGG ACC ACG T 3′ (SEQ. ID NO. 9)5′ GCA TTG ACA GAT GCA CAC G 3′ (SEQ. ID NO. 10) 211 CA 60° C. WMS063 5′TCG ACC TGA TCG CCC CTA 3′ (SEQ. ID NO. 11) 5′ CGC CCT GGG TGA TGA ATAGT 3′ (SEQ. ID NO. 12) 271 GAA,CA,TA 60° C. WMS067 5′ ACC ACA CAA ACAAGG TAA GCG 3′ (SEQ. ID NO. 13) 5′ CAA CCC TCT TAA TTT TGT TGG G 3′(SEQ. ID NO. 14) 85 CA 60° C. WMS068 5′ AGG CCA GAA TCT GGG AAT G 3′(SEQ. ID NO. 15) 5′ CTC CCT AGA TGG GAG AAG GG 3′ (SEQ. ID NO. 16) 182GA 60° C. WMS070 5′ AGT GGC TGG GAG AGT GTC AT 3′ (SEQ. ID NO. 17) 5′GCC CAT TAC CGA GGA CAC 3′ (SEQ. ID NO. 18) 194 GT 60° C. WMS071 5′ GGCAGA GCA GCG ACA CTC 3′ (SEQ. ID NO. 19) 5′ CAA GTG GAG CAT TAG GTA CAC G3′ (SEQ. ID NO. 20) 128 GT 60° C. WMS077 5′ ACA AAC GTA AGC AGC ACC TG3′ (SEQ. ID NO. 21) 5′ ACC CTC TTG CCC GTG TGT TTG 3′ (SEQ. ID NO. 22)153 CA,GA 55° C. WMS082 5′ ACG TTA GAA GGT GCA ATG GG 3′ (SEQ. ID NO.23) 5′ AGT GGA TGC ACC GAC TTT G 3′ (SEQ. ID NO. 24) 152 GT,GAimp 60° C.WMS088 5′ CAC TAC AAC TAT GCG CTC GC 3′ (SEQ. ID NO. 25) 5′ TCC ATT GGCTTC TCT CTC AA 3′ (SEQ. ID NO. 26) 121 GT 60° C. WMS095 5′ GAT CAA ACACAC ACC CCT CC 3′ (SEQ. ID NO. 27) 5′ AAT GCA AAG TGA AAA ACC CG 3′(SEQ. ID NO. 28) 121 CA 60° C. WMS099 5′ AAG ATG GAC GTA TGC ATC ACA 3′(SEQ. ID NO. 29) 5′ GCC ATA TTT GAT GAC GCA TA 3′ (SEQ. ID NO. 30) 119CA 60° C. WMS102 5′ TCT CCC ATC CAA CGC CTC 3′ (SEQ. ID NO. 31) 5′ TGTTGG TGG CTT GAC TAT TG 3′ (SEQ. ID NO. 32) 143 CT 60° C. WMS106 5′ CTGTTC TTG CGT GGC ATT AA 3′ (SEQ. ID NO. 33) 5′ AAT AAG GAC ACA ATT GGGATG G 3′ (SEQ. ID NO. 34) 139 GA 60° C. WMS107 5′ ATT AAT ACC TGA GGGAGC TGC 3′ (SEQ. ID NO. 35) 5′ GGT CTC AGG AGC AAG AAC AC 3′ (SEQ. IDNO. 36) 195 CT 60° C. WMS108 5′ CGA CAA TCG GGT CTT AGC AT 3′ (SEQ. IDNO. 37) 5′ TGC ACA CTT AAA TTA CAT CCG C 3′ (SEQ. ID NO. 38) 132 GTimp60° C. WMS111 5′ TCT GTA GGC TCT CTC CGA CTG 3′ (SEQ. ID NO. 39) 5′ ACCTGA TCA GAT CCC ACT CG 3′ (SEQ. ID NO. 40) 205 CT,GT 55° C. WMS112 5′CTA AAC ACG ACA GCG GTG G 3′ (SEQ. ID NO. 41) 5′ GAT ATG TGA GCA GCG GTCAG 3′ (SEQ. ID NO. 42) 101 CTimp 55° C. WMS113 5′ ATT CGA GGT TAG GAGGAA GAG G 3′ (SEQ. ID NO. 43) 5′ GAG GGT CGG CCT ATA AGA CC 3′ (SEQ. IDNO. 44) 148 GT 60° C. WMS114 5′ ACA AAC AGA AAA TCA AAA CCC G 3′ (SEQ.ID NO. 45) 5′ ATC CAT CGC CAT TGG AGT G 3′ (SEQ. ID NO. 46) 206 GA 60°C. (177) 60° C. WMS118 5′ GAT GTT GCC ACT TGA GCA TG 3′ (SEQ. ID NO. 47)5′ GAT TAG TCA AAT GGA ACA CCC C 3′ (SEQ. ID NO. 48) 110 CA 60° C.WMS119 5′ TGA CTA ACA TCC TTT GTC ACG C 3′ (SEQ. ID NO. 49) 5′ CAT GTCTCA ACC ACC CAC AG 3′ (SEQ. ID NO. 50) 181 GTimp 55° C. WMS120 5′ GATCCA CCT TCC TCT CTC TC 3′ (SEQ. ID NO. 51) 5′ GAT TAT ACT GGT GCC GAA AC3′ (SEQ. ID NO. 52) 139 CT,CA 55° C. WMS121 5′ TCC TCT ACA AAC AAA CACAC 3′ (SEQ. ID NO. 53) 5′ CTC GCA ACT AGA GGT GTA TG 3′ (SEQ. ID NO. 54)143 CA 50° C. WMS122 5′ GGG TGG GAG AAA GGA GAT G 3′ (SEQ. ID NO. 55) 5′AAA CCA TCC TCC ATC CTG G 3′ (SEQ. ID NO. 56) 149 CT,CA 60° C. WMS124 5′GCC ATG GCT ATC ACC CAG 3′ (SEQ. ID NO. 57) 5′ ACT GTT CGG TGC AAT TTGAG 3′ (SEQ. ID NO. 58) 213 CT,GTimp 60° C. WMS126 5′ CAC ACG CTC CAC CATGAC 3′ (SEQ. ID NO. 59) 5′ GTT GAG TTG ATG CGG GAG G 3′ (SEQ. ID NO. 60)196 CA 60° C. WMS128 5′ AGC ACA TTT TAA CAC AGA TA 3′ (SEQ. ID NO. 61)5′ ATC TGT GAA ATT TTG AAA AC 3′ (SEQ. ID NO. 62) 176 CA 50° C. WMS1295′ TCA GTG GGC AAG CTA CAC AG 3′ (SEQ. ID NO. 63) 5′ AAA ACT TAG TAG CCGCGT 3′ (SEQ. ID NO. 64) 221 GTimp 55° C. WMS130 5′ AGC TCT GCT TCA CGAGGA AG 3′ (SEQ. ID NO. 65) 5′ CTC CTC TTT ATA TCG CGT CCC 3′ (SEQ. IDNO. 66) 113 GT 60° C. WMS131 5′ AAT CCC CAC CGA TTC TTC TC 3′ (SEQ. IDNO. 67) 5′ AGT TCG TGG GTC TCT GAT GG 3′ (SEQ. ID NO. 68) 131 CT 60° C.WMS132 5′ TAC CAA ATC GAA ACA CAT CAG G 3′ (SEQ. ID NO. 69) 5′ CAT ATCAAG GTC TCC TTC CCC 3′ (SEQ. ID NO. 70) 119 GA,GAA 60° C. WMS133 5′ ATCTAA ACA AGA CGG CGG TG 3′ (SEQ. ID NO. 71) 5′ ATC TGT GAC AAC CGG TGA GA3′ (SEQ. ID NO. 72) 118 CT 60° C. WMS134 5′ CAT GGA ACT TAG ACA GAT TG3′ (SEQ. ID NO. 73) 5′ CAG TAC TTG GTA CTG AAC AGG 3′ (SEQ. ID NO. 74)111 CA 60° C. WMS135 5′ TGT CAA CAT CGT TTT GAA AAG G 3′ (SEQ. ID NO.75) 5′ ACA CTG TCA ACC TGG CAA TG 3′ (SEQ. ID NO. 76) 143 GA 55° C.WMS136 5′ GAC AGC ACC TTG CCC TTT G 3′ (SEQ. ID NO. 77) 5′ CAT CGG CAACAT GCT CAT C 3′ (SEQ. ID NO. 78) 296 CT 60° C. WMS140 5′ ATG GAG ATATTT GGC CTA CAA C 3′ (SEQ. ID NO. 79) 5′ CTT GAC TTC AAG GCG TGA CA 3′(SEQ. ID NO. 80) 251 CT 55° C. WMS144 5′ TTT GCT GTG GTA CGA AAC ATA C3′ (SEQ. ID NO. 81) 5′ ACT CAC AAA TGT CTA ATA AAA C 3′ (SEQ. ID NO. 82)200 GT 50° C. WMS146 5′ CCA AAA AAA CTG CCT GCA TG 3′ (SEQ. ID NO. 83)5′ CTC TGG CAT TGC TCC TTG G 3′ (SEQ. ID NO. 84) 162 GAimp 60° C. WMS1485′ GTG AGG CAG CAA GAG AGA AA 3′ (SEQ. ID NO. 85) 5′ CAA AGC TTG ACT CAGACC AAA 3′ (SEQ. ID NO. 86) 163 CA 60° C. WMS149 5′ CAT TGT TTT CTG CCTCTA GCC 3′ (SEQ. ID NO. 87) 5′ CTA GCA TCG AAC CTG AAC AAG 3′ (SEQ. IDNO. 88) 161 GA 55° C. WMS153 5′ GAT CTC GTC ACC CGG AAT TC 3′ (SEQ. IDNO. 89) 5′ TGG TAG AGA AGG ACG GAG AG 3′ (SEQ. ID NO. 90) 188 GA 60° C.WMS154 5′ TCA CAG AGA GAG AGG GAG GG 3′ (SEQ. ID NO. 91) 5′ ATG TGT ACATGT TGC CTG CA 3′ (SEQ. ID NO. 92) 102 GA 55° C. WMS155 5′ CAA TCA TTTCCC CCT CCC 3′ (SEQ. ID NO. 93) 5′ AAT CAT TGG AAA TCC ATA TGC C 3′(SEQ. ID NO. 94) 141 CT 60° C. WMS156 5′ CCA ACC GTG CTA TTA GTC ATT C3′ (SEQ. ID NO. 95) 5′ CAA TGC AGG CCC TCC TAA C 3′ (SEQ. ID NO. 96) 277GT 60° C. WMS157 5′ GTC GTC GCG GTA AGC TTG 3′ (SEQ. ID NO. 97) 5′ GAGTGA ACA CAC GAG GCT TG 3′ (SEQ. ID NO. 98) 106 CT 60° C. WMS159 5′ GGGCCA ACA CTG GAA CAC 3′ (SEQ. ID NO. 99) 5′ GCA GAA GCT TGT TGG TAG GC 3′(SEQ. ID NO. 100) 192 GT 60° C. WMS160 5′ TTC AAT TCA GTC TTG GCT TGG 3′(SEQ. ID NO. 101) 5′ CTG CAG GAA AAA AAG TAC ACC C 3′ (SEQ. ID NO. 102)184 GA 60° C. WMS161 5′ GAT CGA GTG ATG GCA GAT GG 3′ (SEQ. ID NO. 103)5′ TGT GAA TTA CTT GGA CCT GG 3′ (SEQ. ID NO. 104) 154 CT 60° C. WMS1625′ AGT GGA TCG ACA AGG CTC TG 3′ (SEQ. ID NO. 105) 5′ AGA AGA AGC AAAGCC TTC CC 3′ (SEQ. ID NO. 106) 208 CA 60° C. WMS163 5′ ACC TCG ACA GACCTG GTA CG 3′ (SEQ. ID NO. 107) 5′ GTC TTT GTC ACC CGA TGG AC 3′ (SEQ.ID NO. 108) 127 CT 55° C. WMS164 5′ ACA TTT CTC CCC CAT CGT C 3′ (SEQ.ID NO. 109) 5′ TTG TAA ACA AAT CGC ATG CG 3′ (SEQ. ID NO. 110) 120 CT55° C. WMS165 5′ TGC AGT GGT CAG ATG TTT CC 3′ (SEQ. ID NO. 111) 5′ CTTTTC TTT CAG ATT GCG CC 3′ (SEQ. ID NO. 112) 199 GA 60° C. WMS169 5′ ACCACT GCA GAG AAC ACA TAC G 3′ (SEQ. ID NO. 113) 5′ GTG CTC TGC TCT AAGTGT GGG 3′ (SEQ. ID NO. 114) 196 GA 60° C. WMS174 5′ GGG TTC CTA TCT GGTAAA TCC C 3′ (SEQ. ID NO. 115) 5′ GAC ACA CAT GTT CCT GCC AC 3′ (SEQ. IDNO. 116) 173 CT 55° C. WMS179 5′ AAG TTG AGT TGA TGC GGG AG 3′ (SEQ. IDNO. 117) 5′ CCA TGA CCA GCA TCC ACT C 3′ (SEQ. ID NO. 118) 181 GT 55° C.WMS180 5′ ATC CGC CTA AGG AAT AGT GT 3′ (SEQ. ID NO. 119) 5′ GAT CGC ACGGGA GAG AGA G 3′ (SEQ. ID NO. 120) 84 CT 50° C. WMS181 5′ TCA TTG GTAATG AGG AGA GA 3′ (SEQ. ID NO. 121) 5′ GAA CCA TTC ATG TGC ATG TC 3′(SEQ. ID NO. 122) 135 GA 50° C. WMS182 5′ TGA TGT AGT GAG CCC ATA GGC 3′(SEQ. ID NO. 123) 5′ TTG CAC ACA GCC AAA TAA GG 3′ (SEQ. ID NO. 124) 165CT 60° C. WMS186 5′ GCA GAG CCT GGT TCA AAA AG 3′ (SEQ. ID NO. 125) 5′CGC CTC TAG CGA GAG CTA TG 3′ (SEQ. ID NO. 126) 140 GA 60° C. WMS189 5′AGG AGC AGC GGA ACG AAC 3′ (SEQ. ID NO. 127) 5′ AGA AAT ACG GAA ACC CACCC 3′ (SEQ. ID NO. 128) 117 CA 55° C. WMS190 5′ GTG CTT GCT GAG CTA TGAGTC 3′ (SEQ. ID NO. 129) 5′ GTG CCA CGT GGT ACC TTT G 3′ (SEQ. ID NO.130) >201 CT,GT 60° C. WMS191 5′ AGA CTG TTG TTT GCG GGC 3′ (SEQ. ID NO.131) 5′ TAG CAC GAC AGT TGT ATG CA 3′ (SEQ. ID NO. 132) 128 CT 60° C.WMS192 5′ GGT TTT CTT TCA GAT TGC GC 3′ (SEQ. ID NO. 133) 5′ CGT TGT CTAATC TTG CCT TGC 3′ (SEQ. ID NO. 134) 191 CT 60° C. WMS193 5′ CTT TGT GCACCT CTC TCT CC 3′ (SEQ. ID NO. 135) 5′ AAT TGT GTT GAT GAT TTG GGG 3′(SEQ. ID NO. 136) 171 CT,CA 60° C. WMS194 5′ GAT CTG CTC TAC TCT CCT CC3′ (SEQ. ID NO. 137) 5′ CGA CGC AGA ACT TAA ACA AG 3′ (SEQ. ID NO. 138)131 CT 50° C. WMS195 5′ AGG TGC CGT CGC GTC TAC 3′ (SEQ. ID NO. 139) 5′ACC CCC CAC GTC AGA GAG 3′ (SEQ. ID NO. 140) 108 CT 60° C. WMS197 5′ GAGAAA GAG GTC TGG AGG TCG 3′ (SEQ. ID NO 141) 5′ CAA AAT GCA CAA GAA TGGAGG 3′ (SEQ. ID NO. 142) 126 CT 60° C. WMS198 5′ TTG AAC CGG AAG GAG TACAG 3′ (SEQ. ID NO. 143) 5′ TCA GTT TAT TTT GGG CAT GTG 3′ (SEQ. ID NO.144) 130 CA 60° C. WMS200 5′ TCA ACG GAA CAG ATG AGC G 3′ (SEQ. ID NO.145) 5′ GAC CTG ATG AGA GCA AGC AC 3′ (SEQ. ID NO. 146) 250 CT 60° C.WMS203 5′ CCC AAA GCA GCG CAA GC 3′ (SEQ. ID NO. 147) 5′ ACC AAT GCT ATCGGC TCG 3′ (SEQ. ID NO. 148) 139 CA,GA 55° C. WMS205 5′ CGA CCC GGT TCACTT CAG 3′ (SEQ. ID NO. 149) 5′ AGT CGC CGT TGT ATA GTG CC 3′ (SEQ. IDNO. 150) 152 CT 60° C. WMS210 5′ TGC ATC AAG AAT AGT GTG GAA G 3′ (SEQ.ID NO. 151) 5′ TGA GAG GAA GGC TCA CAC CTT 3′ (SEQ. ID NO. 152) 192 GA60° C. WMS212 5′ AAG CAA CAT TTG CTG CAA TG 3′ (SEQ. ID NO. 153) 5′ TGCAGT TAA CTT GTT GAA AGG A 3′ (SEQ. ID NO. 154) 104 CT 60° C. WMS213 5′TGC CTG GCT CGT TCT ATC TC 3′ (SEQ. ID NO. 155) 5′ CTA GCT TAG CAC TGTCGC CC 3′ (SEQ. ID NO. 156) 184 GA 60° C. WMS218 5′ CGG CAA ACG GAT ATCGAC 3′ (SEQ. ID NO. 157) 5′ AAC AGT AAC TCT CGC CAT AGC C 3′ (SEQ. IDNO. 158) 149 CT 60° C. WMS219 5′ GAT GAG CGA CAC CTA GCC TC 3′ (SEQ. IDNO. 159) 5′ GGG GTC CGA GTC CAC AAC 3′ (SEQ. ID NO. 160) 181 GAimp 60°C. WMS224 5′ TGA GTC CAG CAC TGC TGC 3′ (SEQ. ID NO. 161) 5′ CAA CAT CCGCTC GTA TTC AA 3′ (SEQ. ID NO. 162) 142 CT 50° C. WMS228 5′ TCA TAT GCACCT CTT TCC TAG G 3′ (SEQ. ID NO. 163) 5′ GTG TGC CAC CTT TGA CGT C 3′(SEQ. ID NO. 164) 210 CT,CA 60° C. WMS231 5′ AGC TCG GGA TGA AGC GTG 3′(SEQ. ID NO. 165) 5′ GAT CCG CCG CTG CGT TT 3′ (SEQ. ID NO. 166) 130GAimp 60° C. WMS232 5′ ATC TCA ACG GCA AGC CG 3′ (SEQ. ID NO. 167) 5′CTG ATG CAA GCA ATC CAC C 3′ (SEQ. ID NO. 168) 141 GA 55° C. WMS233 5′TCA AAA CAT AAA TGT TCA TTG GA 3′ (SEQ. ID NO. 169) 5′ TCA ACC GTG TGTAAT TTT GTC C 3′ (SEQ. ID NO. 170) 261 CT 60° C. WMS234 5′ GAG TCC TGATGT GAA GCT GTT G 3′ (SEQ. ID NO. 171) 5′ CTC ATT GGG GTG TGT ACG TG 3′(SEQ. ID NO. 172) 241 CT,CA 55° C. WMS237 5′ GAA TCA CTT GTG AAG CAT CTGG 3′ (SEQ. ID NO. 173) 5′ CTG GAT GCA TCA CAT CCA AC 3′ (SEQ. ID NO.174) 337 55° C. WMS238 5′ TCG CTT CTA CCG CTC ACC 3′ (SEQ. ID NO. 175)5′ AGT GCC TTG CCG AGG TC 3′ (SEQ. ID NO. 176) 204 CT,GT,GGGT 55° C.WMS241 5′ TCT TCC AAC TAA AGC ATA GC 3′ (SEQ. ID NO. 177) 5′ CTT CCA TGGACT ACA TAC TAG C 3′ (SEQ. ID NO. 178) 146 GA 55° C. WMS242 5′ TCC AAGGCA GTA GGC AGG 3′ (SEQ. ID NO. 179) 5′ TGT TGT TGG CCT GTA TGC AT 3′(SEQ. ID NO. 180) 142 GA 55° C. WMS244 5′ GGC AGC TGA GGC AAT CTG 3′(SEQ. ID NO. 181) 5′ TTT GGA CAT TTC CCA GCG 3′ (SEQ. ID NO. 182) 227CAimp 60° C. WMS245 5′ CAG CGC AGT TAG CTC GC 3′ (SEQ. ID NO. 183) 5′ATC TGT CCA TTC GAG CGC 3′ (SEQ. ID NO. 184) 141 CT 60° C. WMS247 5′ GCAATC TTT TTT CTG ACC ACG 3′ (SEQ. ID NO. 185) 5′ ATG TGC ATG TCG GAC GC3′ (SEQ. ID NO. 186) 158 GA 60° C. WMS248 5′ AGG ACT TCC GCA CCC TG 3′(SEQ. ID NO. 187) 5′ TGG CGT GGT CTA AAT GGA C 3′ (SEQ. ID NO. 188) 185CA 60° C. WMS249 5′ CAA ATG GAT CGA GAA AGG GA 3′ (SEQ. ID NO. 189) 5′CTG CCA TTT TTC TGG ATC TAC C 3′ (SEQ. ID NO. 190) 177 GAimp 60° C.WMS251 5′ CAA CTG GTT GCT ACA CAA GCA 3′ (SEQ. ID NO. 191) 5′ GGG ATGTCT GTT CCA TCT TAG 3′ (SEQ. ID NO. 192) 103 CA 55° C. WMS255 5′ CAA CTGTAC GTA GGT TTC ATT GC 3′ (SEQ. ID NO. 193) 5′ TCT GCC GTA AGT CGC CTC3′ (SEQ. ID NO. 194) 148 GA 55° C. WMS257 5′ AGA GTG CAT GGT GGG ACG 3′(SEQ. ID NO. 195) 5′ CCA AGA CGA TGC TGA AGT CA 3′ (SEQ. ID NO. 196) 192GT 60° C. WMS258 5′ GAT CGC TTC ATC TCT CTC TCT C 3′ (SEQ. ID NO. 197)5′ GTA CAC GCC GTA GGC CC 3′ (SEQ. ID NO. 198) >81 CT 60° C. WMS259 5′AGG GAA AAG ACA TCT TTT TTT TC 3′ (SEQ. ID NO. 199) 5′ CGA CCG ACT TCGGGT TC 3′ (SEQ. ID NO. 200) 105 GA 55° C. WMS260 5′ GCC CCC TTG CAC AAATC 3′ (SEQ. ID NO. 201) 5′ CGC AGC TAC AGG AGG CC 3′ (SEQ. ID NO. 202)157 GA 55° C. WMS261 5′ CTC CCT GTA CGC CTA AGG C 3′ (SEQ. ID NO. 203)5′ CTC GCG CTA CTA GCC ATT G 3′ (SEQ. ID NO. 204) 192 CT 55° C. WMS2635′ TCT GCC GTA AGT CGC CTC 3′ (SEQ. ID NO. 205) 5′ GGT TTC ATT GCT TGCCCT AA 3′ (SEQ. ID NO. 206) 134 CT 55° C. WMS264 5′ GAG AAA CAT GCC GAACAA CA 3′ (SEQ. ID NO. 207) 5′ GCA TGC ATG AGA ATA GGA ACT G 3′ (SEQ. IDNO. 208) 219 CA 60° C. WMS265 5′ TGT TGC GGA TGG TCA CTA TT 3′ (SEQ. IDNO. 209) 5′ GAG TAC ACA TTT GGC CTC TGC 3′ (SEQ. ID NO. 210) 200 CT 55°C. WMS268 5′ AGG GGA TAT GTT GTC ACT CCA 3′ (SEQ. ID NO. 211) 5′ TTA TGTGAT TGC GTA CGT ACC C 3′ (SEQ. ID NO. 212) 241 GAimp 55° C. WMS269 5′TGC ATA TAA ACA GTC ACA CAC CC 3′ (SEQ. ID NO. 213) 5′ TTT GAG CTC CAAAGT GAG TTA GC 3′ (SEQ. ID NO. 214) >148 CA 60° C. WMS271 5′ CAA GAT CGTGGA GCC AGC 3′ (SEQ. ID NO. 215) 5′ AGC TGC TAG CTT TTG GGA CA 3′ (SEQ.ID NO. 216) 162 CT,GA 60° C. WMS272 5′ TGC TCT TTG GCG AAT ATA TGG 3′(SEQ. ID NO. 217) 5′ GTT CAA AAC AAA TTA AAA GGC CC 3′ (SEQ. ID NO. 218)140 CA 55° C. WMS273 5′ ATT GGA CGG ACA GAT GCT TT 3′ (SEQ. ID NO. 219)5′ AGC AGT GAG GAA GGG GAT C 3′ (SEQ. ID NO. 220) 167 GA 55° C. WMS2745′ AAC TTG CAA AAC TGT TCT GA 3′ (SEQ. ID NO. 221) 5′ TAT TTG AAG CGGTTT GAT TT 3′ (SEQ. ID NO. 222) 179 GT 50° C. WMS275 5′ AAT TTT CTT CCTCAC TTA TTC T 3′ (SEQ. ID NO. 223) 5′ AAC AAA AAA TTA GGG CC 3′ (SEQ. IDNO. 224) 107 CT 50° C. WMS276 5′ ATT TGC CTG AAG AAA ATA TT 3′ (SEQ. IDNO. 225) 5′ AAT TTC ACT GCA TAC ACA AG 3′ (SEQ. ID NO. 226) 99 CT 55° C.WMS278 5′ GTT GCT TCA TGA ACG CTC AA 3′ (SEQ. ID NO. 227) 5′ CTG CCC AATTTT CTC CAC TC 3′ (SEQ. ID NO. 228) 241 GTimpGAimp 55° C. WMS281 5′ CGGCCA TAT TTC TGT AAG TAT GC 3′ (SEQ. ID NO. 229) 5′ GCA GGT AAT GGC CGGAC 3′ (SEQ. ID NO. 230) 135 CT 60° C. WMS282 5′ TTG GCC GTG TAA GGC AG3′ (SEQ. ID NO. 231) 5′ TCT CAT TCA CAC ACA ACA CTA GC 3′ (SEQ. ID NO.232) 220 GA 55° C. WMS284 5′ AAT GAA AAA ACA CTT GCG TGG 3′ (SEQ. ID NO.233) 5′ GCA CAT TTT TCA CTT TCG GG 3′ (SEQ. ID NO. 234) 123 GA 60° C.WMS285 5′ ATG ACC CTT CTG CCA AAC AC 3′ (SEQ. ID NO. 235) 5′ ATC GAC CGGGAT CTA GCC 3′ (SEQ. ID NO. 236) 243 GA 60° C. WMS291 5′ CAT CCC TAC GCCACT CTG C 3′ (SEQ. ID NO. 237) 5′ AAT GGT ATC TAT TCC GAC CCG 3′ (SEQ.ID NO. 238) >158 CA 60° C. WMS292 5′ TCA CCG TGG TCA CCG AC 3′ (SEQ. IDNO. 239) 5′ CCA CCG AGC CGA TAA TGT AC 3′ (SEQ. ID NO. 240) 220 CT 60°C. WMS293 5′ TAC TGG TTC ACA TTG GTG CG 3′ (SEQ. ID NO. 241) 5′ TCG CCATCA CTC GTT CAA G 3′ (SEQ. ID NO. 242) 201 CA 55° C. WMS294 5′ GGA TTGGAG TTA AGA GAG AAC CG 3′ (SEQ. ID NO. 243) 5′ GCA GAG TGA TCA ATG CCAGA 3′ (SEQ. ID NO. 244) 100 GAimp 55° C. WMS295 5′ GTG AAG CAG ACC CACAAC AC 3′ (SEQ. ID NO. 245) 5′ GAC GGC TGC GAC GTA GAG 3′ (SEQ. ID NO.246) 258 GA 60° C. WMS296 5′ AAT TCA ACC TAC CAA TCT CTG 3′ (SEQ. ID NO.247) 5′ GCC TAA TAA ACT GAA AAC GAG 3′ (SEQ. ID NO. 248) 149 CT 55° C.WMS297 5′ ATC GTC ACG TAT TTT GCA ATG 3′ (SEQ. ID NO. 249) 5′ TGC GTAAGT CTA GCA TTT TCT G 3′ (SEQ. ID NO. 250) 150 GT,GA 55° C. WMS299 5′ACT ACT TAG GCC TCC CGC C 3′ (SEQ. ID NO. 251) 5′ TGA CCC ACT TGC AATTCA TC 3′ (SEQ. ID NO. 252) 208 GA,TAG 55° C. WMS301 5′ GAG GAG TAA GACACA TGC CC 3′ (SEQ. ID NO. 253) 5′ GTG GCT GGA GAT TCA GGT TC 3′ (SEQ.ID NO. 254) 204 GA,G 55° C. WMS302 5′ GCA AGA AGC AAC AGC AGT AAC 3′(SEQ. ID NO. 255) 5′ CAG ATG CTC TTC TCT GCT GG 3′ (SEQ. ID NO. 256) 180(340) GA 60° C. WMS304 5′ AGG AAA CAG AAA TAT CGC GG 3′ (SEQ. ID NO.257) 5′ AGG ACT GTG GGG AAT GAA TG 3′ (SEQ. ID NO. 258) 217 CT 55° C.WMS311 5′ TCA CGT GGA AGA CGC TCC 3′ (SEQ. ID NO. 259) 5′ CTA CGT GCACCA CCA TTT TG 3′ (SEQ. ID NO. 260) 151 GA 60° C. WMS312 5′ ATC GCA TGATGC ACG TAG AG 3′ (SEQ. ID NO. 261) 5′ ACA TGC ATG CCT ACC TAA TGG 3′(SEQ. ID NO. 262) 235 GA 60° C. WMS313 5′ CCG CCC TCA TTA AGT TTC AC 3′(SEQ. ID NO. 263) 5′ TTT GAC AAG TAC ACG AGT CTG C 3′ (SEQ. ID NO. 264)156 CT,GT 55° C. WMS314 5′ AGG AGC TCC TCT GTG CCA C 3′ (SEQ. ID NO.265) 5′ TTC GGG ACT CTC TTC CCT G 3′ (SEQ. ID NO. 266) 170 CT 55° C.WMS316 5′ CAT GGA CAT TTT ACC ACA AGA C 3′ (SEQ. ID NO. 267) 5′ TGC GTGTGG TCC ACC TC 3′ (SEQ. ID NO. 268) 176 AT,GT 55° C. WMS319 5′ GGT TGCTGT ACA AGT GTT CAC G 3′ (SEQ. ID NO. 269) 5′ CGG GTG CTG TGT GTA ATG AC3′ (SEQ. ID NO. 270) 200 CT 55° C. WMS320 5′ CGA GAT ACT ATG GAA GGT GAGG 3′ (SEQ. ID NO. 271) 5′ ATC TTT GCA AGG ATT GCC C 3′ (SEQ. ID NO.272) >263 GT,GA 55° C. WMS321 5′ CAA TGT GGA GAC GGT GTG C 3′ (SEQ. IDNO. 273) 5′ TGT TGC ATG CGA TCA TGC 3′ (SEQ. ID NO. 274) 265 GT.GAimp60° C. WMS322 5′ TCA CAA AAT GAT TTC TCA TCC G 3′ (SEQ. ID NO. 275) 5′TGC AGA AAA CCA ACA AGG G 3′ (SEQ. ID NO. 276) 119 GA 55° C. WMS325 5′TTT CTT CTG TCG TTC TCT TCC C 3′ (SEQ. ID NO. 277) 5′ TTT TTA CGC GTCAAC GAC G 3′ (SEQ. ID NO. 278) 131 CF 55° C. WMS328 5′ GCA ATC CAC GAGAAG AGA GG 3′ (SEQ. ID NO. 279) 5′ CAC AAA CTC TTG ACA TGT GCG 3′ (SEQ.ID NO. 280) 193 GT 55° C. WMS330 5′ TTG CTA TCC ATG TGC CAG AG 3′ (SEQ.ID NO. 281) 5′ ACA TGT TTC ATG CAG GTA GCC 3′ (SEQ. ID NO. 282) 165 GTT55° C. WMS332 5′ AGC CAG CAA GTC ACC AAA AC 3′ (SEQ. ID NO. 283) 5′ AGTGCT GGA AAG AGT AGT GAA GC 3′ (SEQ. ID NO. 284) 231 GA 60° C. WMS333 5′GCC CGG TCA TGT AAA ACG 3′ (SEQ. ID NO. 285) 5′ TTT CAG TTT GCG TTA AGCTTT G 3′ (SEQ. ID NO. 286) 150 GA 55° C. WMS334 5′ AAT TTC AAA AAG GAGAGA GA 3′ (SEQ. ID NO. 287) 5′ AAC ATG TGT TTT TAG CTA TC 3′ (SEQ. IDNO. 288) 123 GA 50° C. WMS335 5′ CGT ACT CCA CTC CAC ACG G 3′ (SEQ. IDNO. 289) 5′ CGG TCC AAG TGC TAC CTT TC 3′ (SEQ. ID NO. 290) 187 (225)GA,GCGT 55° C. WMS336 5′ CCC TTT AAT CTC GCT CCC TC 3′ (SEQ. ID NO. 291)5′ GTC TCT TTC TCG TAC TTC CAG G 3′ (SEQ. ID NO. 292) 108 CT 55° C.WMS337 5′ CCT CTT CCT CCC TCA CTT AGC 3′ (SEQ. ID NO. 293) 5′ TGC TAACTG GCC TTT GCC 3′ (SEQ. ID NO. 294) 183 CT,CACT,CA 55° C. WMS339 5′ AATTTT CTT CCT CAC TTA TT 3′ (SEQ. ID NO. 295) 5′ AAA CGA ACA ACC ACT CAATC 3′ (SEQ. ID NO. 296) 159 CT 60° C. WMS340 5′ GCA ATC TTT TTT CTG ACCACG 3′ (SEQ. ID NO. 297) 5′ ACG AGG CAA GAA CAC ACA TG 3′ (SEQ. ID NO.298) 132 GA 60° C. WMS341 5′ TTC AGT GGT AGC GGT CGA G 3′ (SEQ. ID NO.299) 5′ CCG ACA TCT CAT GGA TCC AC 3′ (SEQ. ID NO. 300) 133 (150) CT 55°C. WMS342 5′ TAT CCA GAG CAG ACG GAC G 3′ (SEQ. ID NO. 301) 5′ GGT CTAGCT TCG ACG ACA CC 3′ (SEQ. ID NO. 302) 169 CT 55° C. WMS344 5′ CAA GGAAAT AGG CGG TAA TT 3′ (SEQ. ID NO. 303) 5′ ATT TGA GTC TGA AGT TTG CA 3′(SEQ. ID NO. 304) 131 CT 55° C. WMS346 5′ CAA GCA AGG TTT CGT TTT ATC C3′ (SEQ. ID NO. 305) 5′ GCA TGT GGT CCA TGT ACT GC 3′ (SEQ. ID NO. 306)203 AT,GT 55° C. WMS349 5′ GGC TTC CAG AAA ACA ACA GG 3′ (SEQ. ID NO.307) 5′ ATC GGT GCG TAC CAT CCT AC 3′ (SEQ. ID NO. 308) 230 GA 55° C.WMS350 5′ ACC TCA TCC ACA TGT TCT ACG 3′ (SEQ. ID NO. 309) 5′ GCA TGGATA GGA CGC CC 3′ (SEQ. ID NO. 310) 146 CT 55° C. WMS353 5′ CCA TGT TGAGTA GGT TCA GCC 3′ (SEQ. ID NO. 311) 5′ CTT GGC CAG AAG CTA CGA AC 3′(SEQ. ID NO. 312) 179 GCCT,CT 60° C. WMS356 5′ AGC GTT CTT GGG AAT TAGAGA 3′ (SEQ. ID NO. 313) 5′ CCA ATC AGC CTG CAA CAA C 3′ (SEQ. ID NO.314) 224 GA 55° C. WMS357 5′ TAT GGT CAA AGT TGG ACC TCG 3′ (SEQ. ID NO.315) 5′ AGG CTG CAG CTC TTC TTC AG 3′ (SEQ. ID NO. 316) 123 GA 55° C.WMS358 5′ AAA CAG CGG ATT TCA TCG AG 3′ (SEQ. ID NO. 317) 5′ TCC GCT GTTGTT CTG ATC TC 3′ (SEQ. ID NO. 318) 164 GAimp 55° C. WMS359 5′ CTA ATTGCA ACA GGT CAT GGG 3′ (SEQ. ID NO. 319) 5′ TAC TTG TGT TCT GGG ACA ATGG 3′ (SEQ. ID NO. 320) 217 CT,CTTimp 55° C. WMS361 5′ GTA ACT TGT TGCCAA AGG GG 3′ (SEQ. ID NO. 321) 5′ ACA AAG TGG CAA AAG GAG ACA 3′ (SEQ.ID NO. 322) 126 GAimp 60° C. WMS368 5′ CCA TTT CAC CTA ATG CCT GC 3′(SEQ. ID NO. 323) 5′ AAT AAA ACC ATG AGC TCA CTT GC 3′ (SEQ. ID NO. 324)249 AT 60° C. WMS369 5′ CTG CAG GCC ATG ATG ATG 3′ (SEQ. ID NO. 325) 5′ACC GTG GCT GTT GTG AGC 3′ (SEQ. ID NO. 326) 188 CTimp 60° C. WMS371 5′GAC CAA GAT ATT CAA ACT GGC C 3′ (SEQ. ID NO. 327) 5′ AGC TCA GCT TGCTTG GTA CC 3′ (SEQ. ID NO. 328) 170 CA,GA 60° C. WMS372 5′ AAT AGA GCCCTG GGA CTG GG 3′ (SEQ. ID NO. 329) 5′ GAA GGA CGA CAT TCC ACC TG 3′(SEQ. ID NO. 330) >329 GA 60° C. WMS374 5′ ATA GTG TGT TGC ATG CTG TGT G3′ (SEQ. ID NO. 331) 5′ TCT AAT TAG CGT TGG CTG CC 3′ (SEQ. ID NO. 332)213 GT 60° C. WMS375 5′ ATT GGC GAC TCT AGC ATA TAC G 3′ (SEQ. ID NO.333) 5′ GGG ATG TCT GTT CCA TCT TAG C 3′ (SEQ. ID NO. 334) 156 CA 55° C.WMS376 5′ GGG CTA GAA AAC AGG AAG GC 3′ (SEQ. ID NO. 335) 5′ TCT CCC GGAGGG TAG GAG 3′ (SEQ. ID NO. 336) 147 CA,GAimp 60° C. WMS382 5′ GTC AGATAA CGC CGT CCA AT 3′ (SEQ. ID NO. 337) 5′ CTA CGT GCA CCA CCA TTT TG 3′(SEQ. ID NO. 338) 115 GA 60° C. WMS383 5′ ACG CCA GTT GAT CCG TAA AC 3′(SEQ. ID NO. 339) 5′ GAC ATC AAT AAC CGT GGA TGG 3′ (SEQ. ID NO. 340)195 GT 60° C. WMS384 5′ TTT TCA TTG TGC CCT CTA CT 3′ (SEQ. ID NO. 341)5′ GCC AAG TTT CTT AGC TAG TTA A 3′ (SEQ. ID NO. 342) 204 GTimp 55° C.WMS388 5′ CTA CAA TTC GAA GGA GAG GGG 3′ (SEQ. ID NO. 343) 5′ CAC CGCGTC AAC TAC TTA AGC 3′ (SEQ. ID NO. 344) 162 CT,CA,CA, 60° C. WMS389 5′ATC ATG TCG ATC TCC TTG ACG 3′ (SEQ. ID NO. 345) 5′ TGC CAT GCA CAT TAGCAG AT 3′ (SEQ. ID NO. 346) 130 CT,GT 60° C. WMS390 5′ AAG TTT CAC ACAAGA TCT CTC C 3′ (SEQ. ID NO. 347) 5′ TGA CAA GTA CAC GAG TCT GC 3′(SEQ. ID NO. 348) 143 CT,GT 55° C. WMS391 5′ ATA GCG AAG TCT CCC TAC TCCA 3′ (SEQ. ID NO. 349) 5′ ATG TGC ATG TCG GAC GC 3′ (SEQ. ID NO. 350)150 CA,GA 55° C. WMS393 5′ TCA TCT GCT ATT TGT GCT ACA 3′ (SEQ. ID NO.351) 5′ TCA AAT ACA CCA ATG TGC C3′ (SEQ. ID NO. 352) 107 CA 55° C.WMS395 5′ TAC AAC CGC AAG TAA TGC CA 3′ (SEQ. ID NO. 353) 5′ TAC CAA CACCCT AGC CCT TG 3′ (SEQ. ID NO. 354) 147 CA 60° C. WMS397 5′ TGT CAT GGATTA TTT TGG TCG G 3′ (SEQ. ID NO. 355) 5′ CTG CAC TCT CGG TAT ACC AGC 3′(SEQ. ID NO. 356) 179 CT 55° C. WMS400 5′ GTG CTG CCA CCA CTT GC 3′(SEQ. ID NO. 357) 5′ TGT AGG CAC TGC TTG GGA G 3′ (SEQ. ID NO. 358) 139CA 60° C. WMS403 5′ CGA CAT TGG CTT CGG TG 3′ (SEQ. ID NO. 359) 5′ ATAAAA CAG TGC GGT CCA GG 3′ (SEQ. ID NO. 360) 133 CA 55° C. WMS408 5′ TCGATT TAT TTG GGC CAC TG 3′ (SEQ. ID NO. 361) 5′ GTA TAA TTC GTT CAC AGCACG C 3′ (SEQ. ID NO. 362) 176 CA 55° C. WMS410 5′ GCT TGA GAC CGG CACAGT 3′ (SEQ. ID NO. 363) 5′ CGA GAC CTT GAG GGT CTA GA 3′ (SEQ. ID NO.364) 334 CA 55° C. WMS411 5′ CCC ATA CGA TGA TGT GTT TCC 3′ (SEQ. ID NO.365) 5′ CAA ACG GAA CAT GGT CCC 3′ (SEQ. ID NO. 366) 148 CT 55° C.WMS412 5′ ATC AAC AAG GTT TGT GTG TTG G 3′ (SEQ. ID NO. 367) 5′ ATG AAACGC GAC CTC CC 3′ (SEQ. ID NO. 368) 121 GA 55° C. WMS413 5′ TGC TTG TCTAGA TTG CTT GGG 3′ (SEQ. ID NO. 369) 5′ GAT CGT CTC GTC CTT GGC A 3′(SEQ. ID NO. 370) 94 GA 60° C. WMS415 5′ GAT CTC CCA TGT CCG CC 3′ (SEQ.ID NO. 371) 5′ CGA CAG TCG TCA CTT GCC TA 3′ (SEQ. ID NO. 372) 131 GAimp55° C. WMS425 5′ GAG CCC ACA AGC TGG CA 3′ (SEQ. ID NO. 373) 5′ TCG TTCTCC CAA GGC TTG 3′ (SEQ. ID NO. 374) >143 CT 60° C. WMS427 5′ AAA CTTAGA ACT GTA ATT TCA GA 3′ (SEQ. ID NO. 375) 5′ AGT GTG TTC ATT TGA CAGTT 3′ (SEQ. ID NO. 376) 215 CA 50° C. WMS428 5′ CGA GGC AGC GAG GAT TT3′ (SEQ. ID NO. 377) 5′ TTC TCC ACT AGC CCC GC 3′ (SEQ. ID NO. 378) 143GA 60° C. WMS429 5′ TTG TAC ATT AAG TTC CCA T 3′ (SEQ. ID NO. 379) 5′TTT AAG GAC CTA CAT GAC AC 3′ (SEQ. ID NO. 380) 221 (290) CT 50° C.WMS434 5′ ATG AGT TCC GCC AAA GAA TG 3′ (SEQ. ID NO. 381) 5′ ACG AAA TACACA AGT GGG ACA 3′ (SEQ. ID NO. 382) 216 GT 55° C. WMS437 5′ GAT CAA GACTTT TGT ATC TCT C 3′ (SEQ. ID NO. 383) 5′ GAT GTC CAA CAG TTA GCT TA 3′(SEQ. ID NO. 384) 109 CT 50° C. WMS440 5′ CCT ATG GTC TCC ATC ATG AGG 3′(SEQ. ID NO. 385) 5′ TCA TGT CAA CTC AAG AAC ACG 3′ (SEQ. ID NO. 386)112 CT 50° C. WMS443 5′ GGG TCT TCA TCC GGA ACT CT 3′ (SEQ. ID NO. 387)5′ CCA TGA TTT ATA AAT TCC ACC 3′ (SEQ. ID NO. 388) 134 CA,GA 55° C.WMS445 5′ TTT GTT GGG GGT TAG GAT TAG 3′ (SEQ. ID NO. 389) 5′ CCT TAACAC TTG CTG GTA GTG A 3′ (SEQ. ID NO. 390) 192 CT 55° C. WMS448 5′ AAACCA TAT TGG GAG GAA AGG 3′ (SEQ. ID NO. 391) 5′ CAC ATG GCA TCA CAT TTGTG 3′ (SEQ. ID NO. 392) 231 GA 60° C. WMS455 5′ ATT CGG TTC GCT AGC TACCA 3′ (SEQ. ID NO. 393) 5′ ACG GAG AGC AAC CTG CC 3′ (SEQ. ID NO. 394)151 GTimp 55° C. WMS456 5′ TCT GAA CAT TAC ACA ACC CTG A 3′ (SEQ. ID NO.395) 5′ TGC TCT CTC TGA ACC TGA AGC 3′ (SEQ. ID NO. 396) 132 GA 55° C.WMS458 5′ AAT GGC AAT TGG AAG ACA TAG C 3′ (SEQ. ID NO. 397) 5′ TTC GCAATG TTG ATT TGG C 3′ (SEQ. ID NO. 398) 113 CA 60° C. WMS459 5′ ATG GAGTGG TCA CAC TTT GAA 3′ (SEQ. ID NO. 399) 5′ AGC TTC TCT GAC CAA CTT CTCG 3′ (SEQ. ID NO. 400) >138 GA 55° C. WMS469 5′ CAA CTC AGT GCT CAC ACAACG 3′ (SEQ. ID NO. 401) 5′ CGA TAA CCA CTC ATC CAC ACC 3′ (SEQ. ID NO.402) >156 Cr 60° C. WMS471 5′ CGG CCC TAT CAT GGC TG 3′ (SEQ. ID NO.403) 5′ GCT TGC AAG TTC CAT TTT GC 3′ (SEQ. ID NO. 404) 149 CA 60° C.WMS473 5′ TCA TAC GGG TAT GGT TGG AC 3′ (SEQ. ID NO. 405) 5′ CAC CCC CTTGTT GGT CAC 3′ (SEQ. ID NO. 406) 220 GTimp 55° C. WMS476 5′ ATG GGT TCGTAC TAA CAT CAG C 3′ (SEQ. ID NO. 407) 5′ TTG CTG GTA GCT TCA ATC CC 3′(SEQ. ID NO. 408) >194 GAimp 60° C. WMS480 5′ TGC TGC TAC TTG TAC AGAGGA C 3′ (SEQ. ID NO. 409) 5′ CCG AAT TGT CCG CCA TAG 3′ (SEQ. ID NO.410) 188 CT,CA 60° C. WMS484 5′ ACA TCG CTC TTC ACA AAC CC 3′ (SEQ. IDNO. 411) 5′ AGT TCC GGT CAT GGC TAG G 3′ (SEQ. ID NO. 412) 145 CT 55° C.WMS494 5′ ATT GAA CAG GAA GAC ATC AGG G 3′ (SEQ. ID NO. 413) 5′ TTC CTGGAG CTG TCT GGC 3′ (SEQ. ID NO. 414) 198 CA 60° C. WMS495 5′ GAG AGC CTCGCG AAA TAT AGG 3′ (SEQ. ID NO. 415) 5′ TGC TTC TGG TGT TCC TTC G 3′(SEQ. ID NO. 416) 168 GA 60° C. WMS497 5′ GTA GTG AAG ACA AGG GCA TT 3′(SEQ. ID NO. 417) 5′ CCG AAA GTT GGG TGA TAT AC 3′ (SEQ. ID NO.418) >106 GTimp 55° C. WMS499 5′ ACT TGT ATG CTC CAT TGA TTG G 3′ (SEQ.ID NO. 419) 5′ GGG GAG TGG AAA CTG CAT AA 3′ (SEQ. ID NO. 420) 145 GA60° C. WMS501 5′ GGC TAT CTC TGG CGC TAA AA 3′ (SEQ. ID NO. 421) 5′ TCCACA AAC AAG TAG CGC C 3′ (SEQ. ID NO. 422) 172 CA 60° C. WMS512 5′ AGCCAC CAT CAG CAA AAA TT 3′ (SEQ. ID NO. 423) 5′ GAA CAT GAG CAG TTT GGCAC 3′ (SEQ. ID NO. 424) 185 CT 60° C. WMS513 5′ ATC CGT AGC ACC TAC TGGTCA 3′ (SEQ. ID NO. 425) 5′ GGT CTG TTC ATG CCA CAT TG 3′ (SEQ. ID NO.426) 144 CA 60° C. WMS515 5′ AAC ACA ATG GCA AAT GCA GA 3′ (SEQ. ID NO.427) 5′ CCT TCC TAG TAA GTG TGC CTC A 3′ (SEQ. ID NO. 428) 134 GTimp 60°C. WMS518 5′ AAT CAC AAC AAG GCG TGA CA 3′ (SEQ. ID NO. 429) 5′ CAG GGTGGT GCA TGC AT 3′ (SEQ. ID NO. 430) 166 CA 55° C. WMS530 5′ AAA TAG GACAAC CCA CGG C 3′ (SEQ. ID NO. 431) 5′ TCA ACT TCT TGG CCT CCA TC 3′(SEQ. ID NO. 432) 186 CT 55° C. WMS532 5′ ACT GCG TGT GCC TAC AAT TG 3′(SEQ. ID NO. 433) 5′ TCA CTC GCA CTC GAT AGG C 3′ (SEQ. ID NO. 434) 142GT 60° C. WMS533 5′ AAG GCG AAT CAA ACG GAA TA 3′ (SEQ. ID NO. 435) 5′GTT GCT TTA GGG GAA AAG CC 3′ (SEQ. ID NO. 436) 147 CT,CA 60° C. WMS5375′ ACA TAA TGC TTC CTG TGC ACC 3′ (SEQ. ID NO. 437) 5′ GCC ACT TTT GTGTCG TTC CT 3′ (SEQ. ID NO. 438) 209 CA,TA 60° C. WMS538 5′ GCA TTT CGGGTG AAC CC 3′ (SEQ. ID NO. 439) 5′ GTT GCA TGT ATA CGT TAA GCG G 3′(SEQ. ID NO. 440) 147 GTimp 60° C. WMS540 5′ TCT CGC TGT GAA ATC CTA TTTC 3′ (SEQ. ID NO. 441) 5′ AGG CAT GGA TAG AGG GGC 3′ (SEQ. ID NO. 442)129 CTimp 55° C. WMS544 5′ TAG AAT TCT TTA TGG GCT CTG C 3′ (SEQ. ID NO.443) 5′ AGG ATT CCA ATC CTT CAA AAT T 3′ (SEQ. ID NO. 444) 167CT,ATCT,CT 55° C. WMS550 5′ CCC ACA AGA ACC TTT GAA GA 3′ (SEQ. ID NO.445) 5′ CAT TGT GTG TGC AAG GCA C 3′ (SEQ. ID NO. 446) 150 CT,GT 55° C.WMS554 5′ TGC CCA CAA CGG AAC TTG 3′ (SEQ. ID NO. 447) 5′ GCA ACC ACCAAG CAC AAA GT 3′ (SEQ. ID NO. 448) 160 CT,GTimp 60° C. WMS565 5′ GCGTCA GAT ATG CCT ACC TAG G 3′ (SEQ. ID NO. 449) 5′ AGT GAG TTA GCC CTGAGC CA 3′ (SEQ. ID NO. 450) 142 CA 60° C. WMS566 5′ TCT GTC TAC CCA TGGGAT TTG 3′ (SEQ. ID NO. 451) 5′ CTG GCT TCG AGG TAA GCA AC 3′ (SEQ. IDNO. 452) 130 CA,TA 60° C. WMS569 5′ GGA AAC TTA TTG ATT GAA AT 3′ (SEQ.ID NO. 453) 5′ TCA ATT TTG ACA GAA GAA TT 3′ (SEQ. ID NO. 454) 134 GT47° C. WMS570 5′ TCG CCT TTT ACA GTC GGC 3′ (SEQ. ID NO. 455) 5′ ATG GGTAGC TGA GAG CCA AA 3′ (SEQ. ID NO. 456) 143 CT,GT 60° C. WMS573 5′ AAGAGA TAA CAT GCA AGA AA 3′ (SEQ. ID NO. 457) 5′ TTC AAA TAT GTG GGA ACTAC 3′ (SEQ. ID NO. 458) 212 CA 50° C. WMS577 5′ ATG GCA TAA TTT GGT GAAATT G 3′ (SEQ. ID NO. 459) 5′ TGT TTC AAG CCC AAC TTC TAT T 3′ (SEQ. IDNO. 460) 133 CA,TA 55° C. WMS582 5′ AAG CAC TAC GAA AAT ATG AC 3′ (SEQ.ID NO. 461) 5′ TCT TAA GGG GTG TTA TCA TA 3′ (SEQ. ID NO. 462) 151 CA50° C. WMS583 5′ TTC ACA CCC AAC CAA TAG CA 3′ (SEQ. ID NO. 463) 5′ TCTAGG CAG ACA CAT GCC TG 3′ (SEQ. ID NO. 464) 165 CA 60° C. WMS588 5′ GATCCC CAA TTG CAT GTT G 3′ (SEQ. ID NO. 465) 5′ CTT GCA ACT GGG GGA CAC 3′(SEQ. ID NO. 466) 102 GT 60° C.

[0008] These markers are distinguished by a high degree of polymorphismbetween different wheat varieties or lines and usually detect severalalleles per genetic locus in different wheat lines.

[0009] They can therefore be used for DNA fingerprinting, speciesidentification, relationship or similarity studies, characterization ofcytological lines, such as deletion lines, substitution lines, additionlines, etc. and all forms of genetic mappings, including mapping ofindividual genes and quantitative distinguishing features (QTLs). Inaddition, their use is also very suitable for automation and it ispossible to carry out the detection of the products with nonradioactivemethods.

[0010] With the help of this inventive marker, the possibility isprovided, for example, of differentiating almost all European wheatlines.

[0011] The invention is described in greater detail below by means ofexamples.

1. Amplification of the Microsatellite Markers

[0012] The microsatellite markers are amplified according to thefollowing protocol:

[0013] 10 mM tris-HCl, pH 8

[0014] 50 mM KCl

[0015] 1.5 mM MgCl₂ (in a few exceptional cases 3 mM MgCl₂)

[0016] 0.01% (w/v) gelatin

[0017] 0.2 mM of each desoxynucleotide

[0018] 250 nM of each primer (in each case to the left and right of apair)

[0019] 1-2 units taq polymerase

[0020] 50-150 ng matrixes (template) DNA

[0021] are amplified in a volume of 25 or 50 μL according to thefollowing profile: 92° C.  3 minute 92° C.  1 minute (denaturing phase)60° C.  1 minutes (annealing phase) 45 cycles 72° C.  2 minutes(elongation phase) 72° C. 10 minutes (extension phase)

[0022] The amplification takes place in a Perkin Elmer 9600 with lidheating or in an MJ Research Thermocycler without lid heating. In thisapparatus, a layer of mineral oil is placed over the reactions. Thetemperature of the annealing phase depends on the melting point (T_(m))of the primer and in some cases even is 50° C. or 55° C.

2. Separation of the Microsatellite Markers on Polyacrylamide Gels,Which Are Not Denaturing

[0023] The PCR reactions are mixed with {fraction (1/10)} volume of stopbuffer (0.02 M tris acetate of pH 8.1, 0.025 M sodium acetate, 0.02 MEDTA, 70% glycerin, 0.2% SDS, 0.6% bromphenol blue, 0.6% xylene cyanol)and in each case 25 μL are separated in 10% polyacrylamide gels (1.5 mmthick, 18 cm long).

[0024] Formulation for polyacrylamide gel (10%): 25 mL stock acrylamidesolution (19 g acrylamide, 1 g bisacrylamide, diluted to 100 mL withwater) 10 mL 5 × TBE (1 × TBE = 0.09 M tris borate of pH 8.3, 15 mL0.002 M EDTA water

[0025] are mixed and the polymerization is started by the addition of220 μL of ammonium persulfate (10%, freshly prepared) and 20 μL ofTEMED. Immediately after the addition, the mixture is poured into thesealed gel mold and the comb for forming pockets is inserted. Thepolymerization is completed after about 1 hour. The gel is placed in thegel chamber and a preliminary run is carried out without samples forabout 30 minutes at 150 volts in 1× TBE. After that, the samples areloaded (25 μL of each) and the separation is carried out for 14-16 hoursat 100 volts.

[0026] After the electrophoresis is completed, the gel is stained forabout minutes in ethidium bromide (1-2 drops of 10 mg/mL in 1 liter ofwater) and the fragments are made visible by a UV transilluminator anddocumented.

3. Separation of Microsatellite Markers on Denaturing Gels

[0027] For the separation of the amplified fragments on denaturing gels,an automatic laser fluorescence (A.L.F.) sequencer (Pharmacia), forexample, is used. In order to enable the fragments to be detected bymeans of a laser, one primer per pair is marked at the 5′ end withfluorescein. Per PCR reaction, 0.3 to 1.5 microliters are mixed with 2.5microliters of stop buffer (deionized formamide; 5 mg/mL dextran blue),denatured (1 minute; 90° C.) and loaded onto the gel. Gel plates with a9 cm separation distance are used, as recommended by the manufacturerfor the fragment analysis. The gel solution contains 6.5% Long-Ranger(AT Biochem), 7M urea and 1.2× TBE buffer. The gels are 0.35 or 0.5 mmthick. The conditions for the gel run are 600 V, 40 mA, 50 W, 0.84 sdata collection interval and 2 mW laser energy. The gel runs are endedafter about 80 to 90 minuutes. This is sufficient for detectingfragments up to a size of 300 bp. A gel can be used for four or fiveruns. For each gel run, a data set is obtained. With this data set andby means of internal size standards, the exact fragment sizes aredetermined in the computer program Fragment Manager (Pharmacia) and thusthe smallest size differences of a base pair are determined.

1 466 1 20 DNA Triticum sp. 1 ctatgaggcg gaggttgaag 20 2 18 DNA Triticumsp. 2 tgcggtgctc ttccattt 18 3 21 DNA Triticum sp. 3 gcatctggtacactagctgc c 21 4 20 DNA Triticum sp. 4 tcatggatgc atcacatcct 20 5 21DNA Triticum sp. 5 tcgattctga aaggttcatc g 21 6 21 DNA Triticum sp. 6cgatcaagta gttgaaagcg c 21 7 21 DNA Triticum sp. 7 tctgatcccg tgagtgtaaca 21 8 22 DNA Triticum sp. 8 gaaaaaaatt gcatatgagc cc 22 9 19 DNATriticum sp. 9 tgtcctacac ggaccacgt 19 10 19 DNA Triticum sp. 10gcattgacag atgcacacg 19 11 18 DNA Triticum sp. 11 tcgacctgat cgccccta 1812 20 DNA Triticum sp. 12 cgccctgggt gatgaatagt 20 13 21 DNA Triticumsp. 13 accacacaaa caaggtaagc g 21 14 22 DNA Triticum sp. 14 caaccctcttaattttgttg gg 22 15 19 DNA Triticum sp. 15 aggccagaat ctgggaatg 19 16 20DNA Triticum sp. 16 ctccctagat gggagaaggg 20 17 20 DNA Triticum sp. 17agtggctggg agagtgtcat 20 18 18 DNA Triticum sp. 18 gcccattacc gaggacac18 19 18 DNA Triticum sp. 19 ggcagagcag cgagactc 18 20 22 DNA Triticumsp. 20 caagtggagc attaggtaca cg 22 21 20 DNA Triticum sp. 21 acaaaggtaagcagcacctg 20 22 18 DNA Triticum sp. 22 accctcttgc ccgtgttg 18 23 20 DNATriticum sp. 23 acgttagaag gtgcaatggg 20 24 19 DNA Triticum sp. 24agtggatgca ccgactttg 19 25 20 DNA Triticum sp. 25 cactacaact atgcgctcgc20 26 20 DNA Triticum sp. 26 tccattggct tctctctcaa 20 27 20 DNA Triticumsp. 27 gatcaaacac acacccctcc 20 28 20 DNA Triticum sp. 28 aatgcaaagtgaaaaacccg 20 29 21 DNA Triticum sp. 29 aagatggacg tatgcatcac a 21 30 20DNA Triticum sp. 30 gccatatttg atgacgcata 20 31 18 DNA Triticum sp. 31tctcccatcc aacgcctc 18 32 20 DNA Triticum sp. 32 tgttggtggc ttgactattg20 33 20 DNA Triticum sp. 33 ctgttcttgc gtggcattaa 20 34 22 DNA Triticumsp. 34 aataaggaca caattgggat gg 22 35 21 DNA Triticum sp. 35 attaatacctgagggaggtg c 21 36 20 DNA Triticum sp. 36 ggtctcagga gcaagaacac 20 37 20DNA Triticum sp. 37 cgacaatggg gtcttagcat 20 38 22 DNA Triticum sp. 38tgcacactta aattacatcc gc 22 39 21 DNA Triticum sp. 39 tctgtaggctctctccgact g 21 40 20 DNA Triticum sp. 40 acctgatcag atcccactcg 20 41 19DNA Triticum sp. 41 ctaaacacga cagcggtgg 19 42 20 DNA Triticum sp. 42gatatgtgag cagcggtcag 20 43 22 DNA Triticum sp. 43 attcgaggtt aggaggaagagg 22 44 20 DNA Triticum sp. 44 gagggtcggc ctataagacc 20 45 22 DNATriticum sp. 45 acaaacagaa aatcaaaacc cg 22 46 19 DNA Triticum sp. 46atccatcgcc attggagtg 19 47 20 DNA Triticum sp. 47 gatgttgcca cttgagcatg20 48 22 DNA Triticum sp. 48 gattagtcaa atggaacacc cc 22 49 22 DNATriticum sp. 49 tgactaacat cctttgtcac gc 22 50 20 DNA Triticum sp. 50catgtctcaa ccacccacag 20 51 20 DNA Triticum sp. 51 gatccacctt cctctctctc20 52 20 DNA Triticum sp. 52 gattatactg gtgccgaaac 20 53 20 DNA Triticumsp. 53 tcctctacaa acaaacacac 20 54 20 DNA Triticum sp. 54 ctcgcaactagaggtgtatg 20 55 19 DNA Triticum sp. 55 gggtgggaga aaggagatg 19 56 19DNA Triticum sp. 56 aaaccatcct ccatcctgg 19 57 18 DNA Triticum sp. 57gccatggcta tcacccag 18 58 20 DNA Triticum sp. 58 actgttcggt gcaatttgag20 59 18 DNA Triticum sp. 59 cacacgctcc accatgac 18 60 19 DNA Triticumsp. 60 gttgagttga tgcgggagg 19 61 20 DNA Triticum sp. 61 agcacattttaacacagata 20 62 20 DNA Triticum sp. 62 atctgtgaaa ttttgaaaac 20 63 20DNA Triticum sp. 63 tcagtgggca agctacacag 20 64 18 DNA Triticum sp. 64aaaacttagt agccgcgt 18 65 20 DNA Triticum sp. 65 agctctgctt cacgaggaag20 66 21 DNA Triticum sp. 66 ctcctcttta tatcgcgtcc c 21 67 20 DNATriticum sp. 67 aatccccacc gattcttctc 20 68 20 DNA Triticum sp. 68agttcgtggg tctctgatgg 20 69 22 DNA Triticum sp. 69 taccaaatcg aaacacatcagg 22 70 21 DNA Triticum sp. 70 catatcaagg tctccttccc c 21 71 20 DNATriticum sp. 71 atctaaacaa gacggcggtg 20 72 20 DNA Triticum sp. 72atctgtgaca accggtgaga 20 73 21 DNA Triticum sp. 73 catggaactt agacagaattg 21 74 21 DNA Triticum sp. 74 cagtacttgg tactgaacag g 21 75 22 DNATriticum sp. 75 tgtcaacatc gttttgaaaa gg 22 76 20 DNA Triticum sp. 76acactgtcaa cctggcaatg 20 77 19 DNA Triticum sp. 77 gacagcacct tgccctttg19 78 19 DNA Triticum sp. 78 catcggcaac atgctcatc 19 79 22 DNA Triticumsp. 79 atggagatat ttggcctaca ac 22 80 20 DNA Triticum sp. 80 cttgacttcaaggcgtgaca 20 81 22 DNA Triticum sp. 81 tttgctgtgg tacgaaacat ac 22 8222 DNA Triticum sp. 82 actcacaaat gtctaataaa ac 22 83 20 DNA Triticumsp. 83 ccaaaaaaac tgcctgcatg 20 84 19 DNA Triticum sp. 84 ctctggcattgctccttgg 19 85 20 DNA Triticum sp. 85 gtgaggcagc aagagagaaa 20 86 21DNA Triticum sp. 86 caaagcttga ctcagaccaa a 21 87 21 DNA Triticum sp. 87cattgttttc tgcctctagc c 21 88 21 DNA Triticum sp. 88 ctagcatcgaacctgaacaa g 21 89 20 DNA Triticum sp. 89 gatctcgtca cccggaattc 20 90 20DNA Triticum sp. 90 tggtagagaa ggacggagag 20 91 20 DNA Triticum sp. 91tcacagagag agagggaggg 20 92 20 DNA Triticum sp. 92 atgtgtacat gttgcctgca20 93 18 DNA Triticum sp. 93 caatcatttc cccctccc 18 94 22 DNA Triticumsp. 94 aatcattgga aatccatatg cc 22 95 22 DNA Triticum sp. 95 ccaaccgtgctattagtcat tc 22 96 19 DNA Triticum sp. 96 caatgcaggc cctcctaac 19 97 18DNA Triticum sp. 97 gtcgtcgcgg taagcttg 18 98 20 DNA Triticum sp. 98gagtgaacac acgaggcttg 20 99 18 DNA Triticum sp. 99 gggccaacac tggaacac18 100 20 DNA Triticum sp. 100 gcagaagctt gttggtaggc 20 101 21 DNATriticum sp. 101 ttcaattcag tcttggcttg g 21 102 22 DNA Triticum sp. 102ctgcaggaaa aaaagtacac cc 22 103 20 DNA Triticum sp. 103 gatcgagtgatggcagatgg 20 104 20 DNA Triticum sp. 104 tgtgaattac ttggacgtgg 20 10520 DNA Triticum sp. 105 agtggatcga caaggctctg 20 106 20 DNA Triticum sp.106 agaagaagca aagccttccc 20 107 20 DNA Triticum sp. 107 acctcgacagacctggtacg 20 108 20 DNA Triticum sp. 108 gtctttgtca cccgatggac 20 10919 DNA Triticum sp. 109 acatttctcc cccatcgtc 19 110 20 DNA Triticum sp.110 ttgtaaacaa atcgcatgcg 20 111 20 DNA Triticum sp. 111 tgcagtggtcagatgtttcc 20 112 20 DNA Triticum sp. 112 cttttctttc agattgcgcc 20 11322 DNA Triticum sp. 113 accactgcag agaacacata cg 22 114 21 DNA Triticumsp. 114 gtgctctgct ctaagtgtgg g 21 115 22 DNA Triticum sp. 115gggttcctat ctggtaaatc cc 22 116 20 DNA Triticum sp. 116 gacacacatgttcctgccac 20 117 20 DNA Triticum sp. 117 aagttgagtt gatgcgggag 20 11819 DNA Triticum sp. 118 ccatgaccag catccactc 19 119 20 DNA Triticum sp.119 atccgcctaa ggaatagtgt 20 120 19 DNA Triticum sp. 120 gatcgcacgggagagagag 19 121 20 DNA Triticum sp. 121 tcattggtaa tgaggagaga 20 122 20DNA Triticum sp. 122 gaaccattca tgtgcatgtc 20 123 21 DNA Triticum sp.123 tgatgtagtg agcccatagg c 21 124 20 DNA Triticum sp. 124 ttgcacacagccaaataagg 20 125 20 DNA Triticum sp. 125 gcagagcctg gttcaaaaag 20 12620 DNA Triticum sp. 126 cgcctctagc gagagctatg 20 127 18 DNA Triticum sp.127 aggagcagcg gaacgaac 18 128 20 DNA Triticum sp. 128 agaaatacggaaacccaccc 20 129 21 DNA Triticum sp. 129 gtgcttgctg agctatgagt c 21 13019 DNA Triticum sp. 130 gtgccacgtg gtacctttg 19 131 18 DNA Triticum sp.131 agactgttgt ttgcgggc 18 132 22 DNA Triticum sp. 132 tagcacgacagttgtatgca tg 22 133 20 DNA Triticum sp. 133 ggttttcttt cagattgcgc 20134 21 DNA Triticum sp. 134 cgttgtctaa tcttgccttg c 21 135 20 DNATriticum sp. 135 ctttgtgcac ctctctctcc 20 136 21 DNA Triticum sp. 136aattgtgttg atgatttggg g 21 137 20 DNA Triticum sp. 137 gatctgctctactctcctcc 20 138 20 DNA Triticum sp. 138 cgacgcagaa cttaaacaag 20 13918 DNA Triticum sp. 139 aggtgccgtc gcgtctac 18 140 18 DNA Triticum sp.140 accccccacg tcagagag 18 141 21 DNA Triticum sp. 141 gagaaagaggtctggaggtc g 21 142 21 DNA Triticum sp. 142 caaaatgcac aagaatggag g 21143 20 DNA Triticum sp. 143 ttgaaccgga aggagtacag 20 144 21 DNA Triticumsp. 144 tcagtttatt ttgggcatgt g 21 145 19 DNA Triticum sp. 145tcaacggaac agatgagcg 19 146 20 DNA Triticum sp. 146 gacctgatgagagcaagcac 20 147 17 DNA Triticum sp. 147 cccaaagcag cgcaagc 17 148 18DNA Triticum sp. 148 accaatgcta tcggctcg 18 149 18 DNA Triticum sp. 149cgacccggtt cacttcag 18 150 20 DNA Triticum sp. 150 agtcgccgtt gtatagtgcc20 151 22 DNA Triticum sp. 151 tgcatcaaga atagtgtgga ag 22 152 20 DNATriticum sp. 152 tgagaggaag gctcacacct 20 153 20 DNA Triticum sp. 153aagcaacatt tgctgcaatg 20 154 22 DNA Triticum sp. 154 tgcagttaacttgttgaaag ga 22 155 20 DNA Triticum sp. 155 tgcctggctc gttctatctc 20156 20 DNA Triticum sp. 156 ctagcttagc actgtcgccc 20 157 18 DNA Triticumsp. 157 cggcaaacgg atatcgac 18 158 22 DNA Triticum sp. 158 aacagtaactctcgccatag cc 22 159 20 DNA Triticum sp. 159 gatgagcgac acctagcctc 20160 18 DNA Triticum sp. 160 ggggtccgag tccacaac 18 161 18 DNA Triticumsp. 161 tgagtccagc actgctgc 18 162 20 DNA Triticum sp. 162 caacatccgctcgtattcaa 20 163 22 DNA Triticum sp. 163 tcatatgcac ctctttccta gg 22164 19 DNA Triticum sp. 164 gtgtgccacc tttgacgtc 19 165 18 DNA Triticumsp. 165 agctcgggat gaagcgtg 18 166 17 DNA Triticum sp. 166 gatccgccgctgcgttt 17 167 17 DNA Triticum sp. 167 atctcaacgg caagccg 17 168 19 DNATriticum sp. 168 ctgatgcaag caatccacc 19 169 23 DNA Triticum sp. 169tcaaaacata aatgttcatt gga 23 170 22 DNA Triticum sp. 170 tcaaccgtgtgtaattttgt cc 22 171 22 DNA Triticum sp. 171 gagtcctgat gtgaagctgt tg 22172 20 DNA Triticum sp. 172 ctcattgggg tgtgtacgtg 20 173 22 DNA Triticumsp. 173 gaatcacttg tgaagcatct gg 22 174 20 DNA Triticum sp. 174ctggatgcat cacatccaac 20 175 18 DNA Triticum sp. 175 tcgcttctac cgctcacc18 176 17 DNA Triticum sp. 176 agtgccttgc cgaggtc 17 177 20 DNA Triticumsp. 177 tcttccaact aaagcatagc 20 178 22 DNA Triticum sp. 178 cttccatggactacatacta gc 22 179 18 DNA Triticum sp. 179 tccaaggcag taggcagg 18 18020 DNA Triticum sp. 180 tgttgttggc ctgtatgcat 20 181 18 DNA Triticum sp.181 ggcagctgag gcaatctg 18 182 18 DNA Triticum sp. 182 tttggacatttcccagcg 18 183 17 DNA Triticum sp. 183 cagcgcagtt agctcgc 17 184 18 DNATriticum sp. 184 atctgtccat tcgagcgc 18 185 21 DNA Triticum sp. 185gcaatctttt ttctgaccac g 21 186 17 DNA Triticum sp. 186 atgtgcatgtcggacgc 17 187 17 DNA Triticum sp. 187 aggacttccg caccctg 17 188 19 DNATriticum sp. 188 tggcgtggtc taaatggac 19 189 20 DNA Triticum sp. 189caaatggatc gagaaaggga 20 190 22 DNA Triticum sp. 190 ctgccatttttctggatcta cc 22 191 21 DNA Triticum sp. 191 caactggttg ctacacaagc a 21192 21 DNA Triticum sp. 192 gggatgtctg ttccatctta g 21 193 23 DNATriticum sp. 193 caactgtacg taggtttcat tgc 23 194 18 DNA Triticum sp.194 tctgccgtaa gtcgcctc 18 195 18 DNA Triticum sp. 195 agagtgcatggtgggacg 18 196 20 DNA Triticum sp. 196 ccaagacgat gctgaagtca 20 197 22DNA Triticum sp. 197 gatcgcttca tctctctctc tc 22 198 17 DNA Triticum sp.198 gtacacgccg taggccc 17 199 23 DNA Triticum sp. 199 agggaaaagacatctttttt ttc 23 200 17 DNA Triticum sp. 200 cgaccgactt cgggttc 17 20117 DNA Triticum sp. 201 gcccccttgc acaaatc 17 202 17 DNA Triticum sp.202 cgcagctaca ggaggcc 17 203 19 DNA Triticum sp. 203 ctccctgtacgcctaaggc 19 204 19 DNA Triticum sp. 204 ctcgcgctac tagccattg 19 205 18DNA Triticum sp. 205 tctgccgtaa gtcgcctc 18 206 20 DNA Triticum sp. 206ggtttcattg cttgccctaa 20 207 20 DNA Triticum sp. 207 gagaaacatgccgaacaaca 20 208 22 DNA Triticum sp. 208 gcatgcatga gaataggaac tg 22209 20 DNA Triticum sp. 209 tgttgcggat ggtcactatt 20 210 21 DNA Triticumsp. 210 gagtacacat ttggcctctg c 21 211 21 DNA Triticum sp. 211aggggatatg ttgtcactcc a 21 212 22 DNA Triticum sp. 212 ttatgtgattgcgtacgtac cc 22 213 23 DNA Triticum sp. 213 tgcatataaa cagtcacaca ccc23 214 23 DNA Triticum sp. 214 tttgagctcc aaagtgagtt agc 23 215 18 DNATriticum sp. 215 caagatcgtg gagccagc 18 216 20 DNA Triticum sp. 216agctgctagc ttttgggaca 20 217 21 DNA Triticum sp. 217 tgctctttggcgaatatatg g 21 218 23 DNA Triticum sp. 218 gttcaaaaca aattaaaagg ccc 23219 20 DNA Triticum sp. 219 attggacgga cagatgcttt 20 220 19 DNA Triticumsp. 220 agcagtgagg aaggggatc 19 221 20 DNA Triticum sp. 221 aacttgcaaaactgttctga 20 222 20 DNA Triticum sp. 222 tatttgaagc ggtttgattt 20 22322 DNA Triticum sp. 223 aattttcttc ctcacttatt ct 22 224 17 DNA Triticumsp. 224 aacaaaaaat tagggcc 17 225 20 DNA Triticum sp. 225 atttgcctgaagaaaatatt 20 226 20 DNA Triticum sp. 226 aatttcactg catacacaag 20 22720 DNA Triticum sp. 227 gttgcttcat gaacgctcaa 20 228 20 DNA Triticum sp.228 ctgcccaatt ttctccactc 20 229 23 DNA Triticum sp. 229 cggccatatttctgtaagta tgc 23 230 17 DNA Triticum sp. 230 gcaggtaatg gccggac 17 23117 DNA Triticum sp. 231 ttggccgtgt aaggcag 17 232 23 DNA Triticum sp.232 tctcattcac acacaacact agc 23 233 21 DNA Triticum sp. 233 aatgaaaaaacacttgcgtg g 21 234 20 DNA Triticum sp. 234 gcacattttt cactttcggg 20 23520 DNA Triticum sp. 235 atgacccttc tgccaaacac 20 236 18 DNA Triticum sp.236 atcgaccggg atctagcc 18 237 19 DNA Triticum sp. 237 catccctacgccactctgc 19 238 21 DNA Triticum sp. 238 aatggtatct attccgaccc g 21 23917 DNA Triticum sp. 239 tcaccgtggt caccgac 17 240 20 DNA Triticum sp.240 ccaccgagcc gataatgtac 20 241 20 DNA Triticum sp. 241 tactggttcacattggtgcg 20 242 19 DNA Triticum sp. 242 tcgccatcac tcgttcaag 19 243 23DNA Triticum sp. 243 ggattggagt taagagagaa ccg 23 244 20 DNA Triticumsp. 244 gcagagtgat caatgccaga 20 245 20 DNA Triticum sp. 245 gtgaagcagacccacaacac 20 246 18 DNA Triticum sp. 246 gacggctgcg acgtagag 18 247 21DNA Triticum sp. 247 aattcaacct accaatctct g 21 248 21 DNA Triticum sp.248 gcctaataaa ctgaaaacga g 21 249 21 DNA Triticum sp. 249 atcgtcacgtattttgcaat g 21 250 22 DNA Triticum sp. 250 tgcgtaagtc tagcattttc tg 22251 19 DNA Triticum sp. 251 actacttagg cctcccgcc 19 252 20 DNA Triticumsp. 252 tgacccactt gcaattcatc 20 253 20 DNA Triticum sp. 253 gaggagtaagacacatgccc 20 254 20 DNA Triticum sp. 254 gtggctggag attcaggttc 20 25521 DNA Triticum sp. 255 gcaagaagca acagcagtaa c 21 256 20 DNA Triticumsp. 256 cagatgctct tctctgctgg 20 257 20 DNA Triticum sp. 257 aggaaacagaaatatcgcgg 20 258 20 DNA Triticum sp. 258 aggactgtgg ggaatgaatg 20 25918 DNA Triticum sp. 259 tcacgtggaa gacgctcc 18 260 20 DNA Triticum sp.260 ctacgtgcac caccattttg 20 261 20 DNA Triticum sp. 261 atcgcatgatgcacgtagag 20 262 21 DNA Triticum sp. 262 acatgcatgc ctacctaatg g 21 26320 DNA Triticum sp. 263 ccgccctcat taagtttcac 20 264 22 DNA Triticum sp.264 tttgacaagt acacgagtct gc 22 265 19 DNA Triticum sp. 265 aggagctcctctgtgccac 19 266 19 DNA Triticum sp. 266 ttcgggactc tcttccctg 19 267 22DNA Triticum sp. 267 catggacatt ttaccacaag ac 22 268 17 DNA Triticum sp.268 tgcgtgtggt ccacctc 17 269 22 DNA Triticum sp. 269 ggttgctgtacaagtgttca cg 22 270 20 DNA Triticum sp. 270 cgggtgctgt gtgtaatgac 20271 22 DNA Triticum sp. 271 cgagatacta tggaaggtga gg 22 272 19 DNATriticum sp. 272 atctttgcaa ggattgccc 19 273 19 DNA Triticum sp. 273caatgtggag acggtgtgc 19 274 18 DNA Triticum sp. 274 tgttgcatgc gatcatgc18 275 22 DNA Triticum sp. 275 tcacaaaatg atttctcatc cg 22 276 19 DNATriticum sp. 276 tgcagaaaac caacaaggg 19 277 22 DNA Triticum sp. 277tttcttctgt cgttctcttc cc 22 278 19 DNA Triticum sp. 278 tttttacgcgtcaacgacg 19 279 20 DNA Triticum sp. 279 gcaatccacg agaagagagg 20 280 21DNA Triticum sp. 280 cacaaactct tgacatgtgc g 21 281 20 DNA Triticum sp.281 ttgctatcca tgtgccagag 20 282 21 DNA Triticum sp. 282 acatgtttcatgcaggtagc c 21 283 20 DNA Triticum sp. 283 agccagcaag tcaccaaaac 20 28423 DNA Triticum sp. 284 agtgctggaa agagtagtga agc 23 285 18 DNA Triticumsp. 285 gcccggtcat gtaaaacg 18 286 22 DNA Triticum sp. 286 tttcagtttgcgttaagctt tg 22 287 20 DNA Triticum sp. 287 aatttcaaaa aggagagaga 20288 20 DNA Triticum sp. 288 aacatgtgtt tttagctatc 20 289 19 DNA Triticumsp. 289 cgtactccac tccacacgg 19 290 20 DNA Triticum sp. 290 cggtccaagtgctacctttc 20 291 20 DNA Triticum sp. 291 ccctttaatc tcgctccctc 20 29222 DNA Triticum sp. 292 gtctctttct cgtacttcca gg 22 293 21 DNA Triticumsp. 293 cctcttcctc cctcacttag c 21 294 18 DNA Triticum sp. 294tgctaactgg cctttgcc 18 295 20 DNA Triticum sp. 295 aattttcttc ctcacttatt20 296 20 DNA Triticum sp. 296 aaacgaacaa ccactcaatc 20 297 21 DNATriticum sp. 297 gcaatctttt ttctgaccac g 21 298 20 DNA Triticum sp. 298acgaggcaag aacacacatg 20 299 19 DNA Triticum sp. 299 ttcagtggtagcggtcgag 19 300 20 DNA Triticum sp. 300 ccgacatctc atggatccac 20 301 19DNA Triticum sp. 301 tatccagagc agacggacg 19 302 20 DNA Triticum sp. 302ggtctagctt cgacgacacc 20 303 20 DNA Triticum sp. 303 caaggaaataggcggtaact 20 304 20 DNA Triticum sp. 304 atttgagtct gaagtttgca 20 30522 DNA Triticum sp. 305 caagcaaggt ttcgttttat cc 22 306 20 DNA Triticumsp. 306 gcatgtggtc catgtactgc 20 307 20 DNA Triticum sp. 307 ggcttccagaaaacaacagg 20 308 20 DNA Triticum sp. 308 atcggtgcgt accatcctac 20 30921 DNA Triticum sp. 309 acctcatcca catgttctac g 21 310 17 DNA Triticumsp. 310 gcatggatag gacgccc 17 311 21 DNA Triticum sp. 311 ccatgttgagtaggttcagc c 21 312 20 DNA Triticum sp. 312 cttggccaga agctacgaac 20 31321 DNA Triticum sp. 313 agcgttcttg ggaattagag a 21 314 19 DNA Triticumsp. 314 ccaatcagcc tgcaacaac 19 315 21 DNA Triticum sp. 315 tatggtcaaagttggacctc g 21 316 20 DNA Triticum sp. 316 aggctgcagc tcttcttcag 20 31720 DNA Triticum sp. 317 aaacagcgga tttcatcgag 20 318 20 DNA Triticum sp.318 tccgctgttg ttctgatctc 20 319 21 DNA Triticum sp. 319 ctaattgcaacaggtcatgg g 21 320 22 DNA Triticum sp. 320 tacttgtgtt ctgggacaat gg 22321 20 DNA Triticum sp. 321 gtaacttgtt gccaaagggg 20 322 21 DNA Triticumsp. 322 acaaagtggc aaaaggagac a 21 323 20 DNA Triticum sp. 323ccatttcacc taatgcctgc 20 324 23 DNA Triticum sp. 324 aataaaaccatgagctcact tgc 23 325 18 DNA Triticum sp. 325 ctgcaggcca tgatgatg 18 32618 DNA Triticum sp. 326 accgtgggtg ttgtgagc 18 327 22 DNA Triticum sp.327 gaccaagata ttcaaactgg cc 22 328 20 DNA Triticum sp. 328 agctcagcttgcttggtacc 20 329 20 DNA Triticum sp. 329 aatagagccc tgggactggg 20 33020 DNA Triticum sp. 330 gaaggacgac attccacctg 20 331 22 DNA Triticum sp.331 atagtgtgtt gcatgctgtg tg 22 332 20 DNA Triticum sp. 332 tctaattagcgttggctgcc 20 333 22 DNA Triticum sp. 333 attggcgact ctagcatata cg 22334 22 DNA Triticum sp. 334 gggatgtctg ttccatctta gc 22 335 20 DNATriticum sp. 335 gggctagaaa acaggaaggc 20 336 18 DNA Triticum sp. 336tctcccggag ggtaggag 18 337 20 DNA Triticum sp. 337 gtcagataac gccgtccaat20 338 20 DNA Triticum sp. 338 ctacgtgcac caccattttg 20 339 20 DNATriticum sp. 339 acgccagttg atccgtaaac 20 340 21 DNA Triticum sp. 340gacatcaata accgtggatg g 21 341 20 DNA Triticum sp. 341 ttttcattgtgccctctact 20 342 22 DNA Triticum sp. 342 gccaagtttc ttagctagtt aa 22343 21 DNA Triticum sp. 343 ctacaattcg aaggagaggg g 21 344 21 DNATriticum sp. 344 caccgcgtca actacttaag c 21 345 21 DNA Triticum sp. 345atcatgtcga tctccttgac g 21 346 20 DNA Triticum sp. 346 tgccatgcacattagcagat 20 347 22 DNA Triticum sp. 347 aagtttcaca caagatctct cc 22348 20 DNA Triticum sp. 348 tgacaagtac acgagtctgc 20 349 22 DNA Triticumsp. 349 atagcgaagt ctccctactc ca 22 350 17 DNA Triticum sp. 350atgtgcatgt cggacgc 17 351 21 DNA Triticum sp. 351 tcatctgcta tttgtgctaca 21 352 19 DNA Triticum sp. 352 tcaaatacac caatgtgcc 19 353 20 DNATriticum sp. 353 tacaaccgca agtaatgcca 20 354 20 DNA Triticum sp. 354taccaacacc ctagcccttg 20 355 21 DNA Triticum sp. 355 tgtcatggattatttggtcg g 21 356 21 DNA Triticum sp. 356 ctgcactctc ggtataccag c 21357 17 DNA Triticum sp. 357 gtgctgccac cacttgc 17 358 19 DNA Triticumsp. 358 tgtaggcact gcttgggag 19 359 17 DNA Triticum sp. 359 cgacattggcttcggtg 17 360 20 DNA Triticum sp. 360 ataaaacagt gcggtccagg 20 361 20DNA Triticum sp. 361 tcgatttatt tgggccactg 20 362 22 DNA Triticum sp.362 gtataattcg ttcacagcac gc 22 363 18 DNA Triticum sp. 363 gcttgagaccggcacagt 18 364 20 DNA Triticum sp. 364 cgagaccttg agggtctaga 20 365 21DNA Triticum sp. 365 cccatacgat gatgtgtttc c 21 366 18 DNA Triticum sp.366 caaacggaac atggtccc 18 367 22 DNA Triticum sp. 367 atcaacaaggtttgtgtgtt gg 22 368 17 DNA Triticum sp. 368 atgaaacgcg acctccc 17 36921 DNA Triticum sp. 369 tgcttgtcta gattgcttgg g 21 370 19 DNA Triticumsp. 370 gatcgtctcg tccttggca 19 371 17 DNA Triticum sp. 371 gatctcccatgtccgcc 17 372 20 DNA Triticum sp. 372 cgacagtcgt cacttgccta 20 373 17DNA Triticum sp. 373 gagcccacaa gctggca 17 374 18 DNA Triticum sp. 374tcgttctccc aaggcttg 18 375 23 DNA Triticum sp. 375 aaacttagaa ctgtaatttcaga 23 376 20 DNA Triticum sp. 376 agtgtgttca tttgacagtt 20 377 17 DNATriticum sp. 377 cgaggcagcg aggattt 17 378 17 DNA Triticum sp. 378ttctccacta gccccgc 17 379 21 DNA Triticum sp. 379 ttgtacatta agttcccatta 21 380 20 DNA Triticum sp. 380 tttaaggacc tacatgacac 20 381 20 DNATriticum sp. 381 atgagttccg ccaaagaatg 20 382 21 DNA Triticum sp. 382acgaaataca caagtgggac a 21 383 22 DNA Triticum sp. 383 gatcaagacttttgtatctc tc 22 384 20 DNA Triticum sp. 384 gatgtccaac agttagctta 20385 21 DNA Triticum sp. 385 cctatggtct ccatcatgag g 21 386 21 DNATriticum sp. 386 tcatgtcaac tcaagaacac g 21 387 20 DNA Triticum sp. 387gggtcttcat ccggaactct 20 388 21 DNA Triticum sp. 388 ccatgatttataaattccac c 21 389 21 DNA Triticum sp. 389 tttgttgggg gttaggatta g 21390 22 DNA Triticum sp. 390 ccttaacact tgctggtagt ga 22 391 21 DNATriticum sp. 391 aaaccatatt gggaggaaag g 21 392 20 DNA Triticum sp. 392cacatggcat cacatttgtg 20 393 20 DNA Triticum sp. 393 attcggttcgctagctacca 20 394 17 DNA Triticum sp. 394 acggagagca acctgcc 17 395 22DNA Triticum sp. 395 tctgaacatt acacaaccct ga 22 396 21 DNA Triticum sp.396 tgctctctct gaacctgaag c 21 397 22 DNA Triticum sp. 397 aatggcaattggaagacata gc 22 398 19 DNA Triticum sp. 398 ttcgcaatgt tgatttggc 19 39921 DNA Triticum sp. 399 atggagtggt cacactttga a 21 400 22 DNA Triticumsp. 400 agcttctctg accaacttct cg 22 401 21 DNA Triticum sp. 401caactcagtg ctcacacaac g 21 402 21 DNA Triticum sp. 402 cgataaccactcatccacac c 21 403 17 DNA Triticum sp. 403 cggccctatc atggctg 17 404 20DNA Triticum sp. 404 gcttgcaagt tccattttgc 20 405 20 DNA Triticum sp.405 tcatacgggt atggttggac 20 406 18 DNA Triticum sp. 406 cacccccttgttggtcac 18 407 22 DNA Triticum sp. 407 atgggttcgt actaacatca gc 22 40820 DNA Triticum sp. 408 ttgctggtag cttcaatccc 20 409 22 DNA Triticum sp.409 tgctgctact tgtacagagg ac 22 410 18 DNA Triticum sp. 410 ccgaattgtccgccatag 18 411 20 DNA Triticum sp. 411 acatcgctct tcacaaaccc 20 412 19DNA Triticum sp. 412 agttccggtc atggctagg 19 413 22 DNA Triticum sp. 413attgaacagg aagacatcag gg 22 414 18 DNA Triticum sp. 414 ttcctggagctgtctggc 18 415 21 DNA Triticum sp. 415 gagagcctcg cgaaatatag g 21 41619 DNA Triticum sp. 416 tgcttctggt gttccttcg 19 417 20 DNA Triticum sp.417 gtagtgaaga caagggcatt 20 418 20 DNA Triticum sp. 418 ccgaaagttgggtgatatac 20 419 22 DNA Triticum sp. 419 acttgtatgc tccattgatt gg 22420 20 DNA Triticum sp. 420 ggggagtgga aactgcataa 20 421 20 DNA Triticumsp. 421 ggctatctct ggcgctaaaa 20 422 19 DNA Triticum sp. 422 tccacaaacaagtagcgcc 19 423 20 DNA Triticum sp. 423 agccaccatc agcaaaaatt 20 424 20DNA Triticum sp. 424 gaacatgagc agtttggcac 20 425 21 DNA Triticum sp.425 atccgtagca cctactggtc a 21 426 20 DNA Triticum sp. 426 ggtctgttcatgccacattg 20 427 20 DNA Triticum sp. 427 aacacaatgg caaatgcaga 20 42822 DNA Triticum sp. 428 ccttcctagt aagtgtgcct ca 22 429 20 DNA Triticumsp. 429 aatcacaaca aggcgtgaca 20 430 17 DNA Triticum sp. 430 cagggtggtgcatgcat 17 431 19 DNA Triticum sp. 431 aaataggaca acccacggc 19 432 20DNA Triticum sp. 432 tcaacttctt ggcctccatc 20 433 20 DNA Triticum sp.433 actgcgtgtg cctacaattg 20 434 19 DNA Triticum sp. 434 tcactcgcactcgataggc 19 435 20 DNA Triticum sp. 435 aaggcgaatc aaacggaata 20 436 20DNA Triticum sp. 436 gttgctttag gggaaaagcc 20 437 21 DNA Triticum sp.437 acataatgct tcctgtgcac c 21 438 20 DNA Triticum sp. 438 gccacttttgtgtcgttcct 20 439 17 DNA Triticum sp. 439 gcatttcggg tgaaccc 17 440 22DNA Triticum sp. 440 gttgcatgta tacgttaagc gg 22 441 22 DNA Triticum sp.441 tctcgctgtg aaatcctatt tc 22 442 18 DNA Triticum sp. 442 aggcatggatagaggggc 18 443 22 DNA Triticum sp. 443 tagaattctt tatggggtct gc 22 44422 DNA Triticum sp. 444 aggattccaa tccttcaaaa tt 22 445 20 DNA Triticumsp. 445 cccacaagaa cctttgaaga 20 446 19 DNA Triticum sp. 446 cattgtgtgtgcaaggcac 19 447 18 DNA Triticum sp. 447 tgcccacaac ggaacttg 18 448 20DNA Triticum sp. 448 gcaaccacca agcacaaagt 20 449 22 DNA Triticum sp.449 gcgtcagata tgcctaccta gg 22 450 20 DNA Triticum sp. 450 agtgagttagccctgagcca 20 451 21 DNA Triticum sp. 451 tctgtctacc catgggattt g 21 45220 DNA Triticum sp. 452 ctggcttcga ggtaagcaac 20 453 20 DNA Triticum sp.453 ggaaacttat tgattgaaat 20 454 20 DNA Triticum sp. 454 tcaattttgacagaagaatt 20 455 18 DNA Triticum sp. 455 tcgcctttta cagtcggc 18 456 20DNA Triticum sp. 456 atgggtagct gagagccaaa 20 457 20 DNA Triticum sp.457 aagagataac atgcaagaaa 20 458 20 DNA Triticum sp. 458 ttcaaatatgtgggaactac 20 459 22 DNA Triticum sp. 459 atggcataat ttggtgaaat tg 22460 22 DNA Triticum sp. 460 tgtttcaagc ccaacttcta tt 22 461 20 DNATriticum sp. 461 aagcactacg aaaatatgac 20 462 20 DNA Triticum sp. 462tcttaagggg tgttatcata 20 463 20 DNA Triticum sp. 463 ttcacacccaaccaatagca 20 464 20 DNA Triticum sp. 464 tctaggcaga cacatgcctg 20 46519 DNA Triticum sp. 465 gatccccaat tgcatgttg 19 466 18 DNA Triticum sp.466 cttgcaactg ggggacac 18

1. Microsatellite markers (based on hypervariable genome sections) forplants of the Triticum aestivum species, as well as of the TribeTriticeae using the polymerase chain reaction (PCR), characterized inthat a sequence tagged site (STS), which is defined by two specificprimers, which average a length of 20±3 bases and flank a microsatellitesequence, which microsatellite markers are amplified to polymorphisms(PCR products of different length).
 2. The microsatellite markers ofclaim 1 characterized in that the microsatellite sequence is atandem-repetitive n-fold repetition of a di-, tri- or tetranucleotidesequence, in which n≧10.
 3. The microsatellite markers of claim 1,characterized in that the microsatellite sequence is a compositemicrosatellite sequence.
 4. The microsatellite markers of claim 1,characterized in that the microsatellite sequence is an imperfectsequence, in which individual bases are mutated.
 5. The microsatellitemarkers of claim 1, characterized in that the following primer pairswith assigned microsatellite sequences or a number thereof arecontained. WMS Number WMS Primer left WMS Primer Right Repeat TypeWMS-Nummer WMS Primer links WMS Primer rechts Repeat-Type WMS052 5′ CTATGA GGC GGA GGT TGA AG 3′ 5′ TGC GGT GCT CTT CCA TTT 3′ GTimp WMS055 5′GCA TCT GGT ACA CTA GCT GCC 3′ 5′ TCA TGG ATG CAT CAC ATC CT 3′ CTimpWMS057 5′ TCG ATT CTG AAA GGT TCA TCG 3′ 5′ CGA TCA AGT AGT TGA AAG CGC3′ AAAAAimp WMS058 5′ TCT GAT CCC GTG AGT GTA ACA 3′ 5′ GAA AAA AAT TGCATA TGA GCC C 3′ CA WMS060 5′ TGT CCT ACA CGG ACC ACG T 3′ 5′ GCA TTGACA GAT GCA CAC G 3′ CA WMS063 5′ TCG ACC TGA TCG CCC CTA 3′ 5′ CCC CCTGGG TGA TGA ATA GT 3′ GAA,CA,TA WMS067 5′ ACC ACA CAA ACA AGG TAA GCG 3′5′ CAA CCC TCT TAA TTT TGT TGG G 3′ CA WMS068 5′ AGG CCA GAA TCT GGG AATG 3′ 5′ CTC CCT AGA TGG GAG AAG GG 3′ GA WMS070 5′ AGT GGC TGG GAG AGTGTC AT 3′ 5′ GCC CAT TAC CGA GGA CAC 3′ GT WMS071 5′ GGC AGA GCA GCG AGACTC 3′ 5′ CAA GTG GAG CAT TAG GTA CAC G 3′ GT WMS077 5′ ACA AAG GTA AGCAGC ACC TG 3′ 5′ ACC CTC TTG CCC GTG TTG 3′ CA,GA WMS082 5′ ACG TTA GAAGGT GCA ATG GG 3′ 5′ AGT GGA TGC ACC GAC TTT G 3′ GT,GAimp WMS088 5′ CACTAC AAC TAT GCG CTC GC 3′ 5′ TCC ATT GGC TTC TCT CTC AA 3′ GT WMS095 5′GAT CAA ACA CAC ACC CCT CC 3′ 5′ AAT GCA AAG TGA AAA ACC CG 3′ CA WMS0995′ AAG ATG GAC GTA TGC ATC ACA 3′ 5′ GCC ATA TTT GAT GAC GCA TA 3′ CAWMS102 5′ TGT CCC ATC CAA CGC CTC 3′ 5′ TGT TGG TGG CTT GAC TAT TG 3′ CTWMS106 5′ CTG TTC TTG CGT GGC ATT AA 3′ 5′ AAT AAG GAC ACA ATT GGG ATG G3′ GA WMS107 5′ ATT AAT ACC TGA GGG AGG TGC 3′ 5′ GGT CTC AGG AGC AAGAAC AC 3′ CT WMS108 5′ CGA CAA TGG GGT CTT AGC AT 3′ 5′ TGC ACA CTT AAATTA CAT CCG C 3′ GTimp WMS111 5′ TCT GTA GGC TCT CTC CGA CTG 3′ 5′ ACCTGA TCA GAT CCC ACT CG 3′ CT,GT WMS112 5′ CTA AAC ACG ACA GCG GTG G 3′5′ GAT ATG TGA GCA GCG GTC AG 3′ CTimp WMS113 5′ ATT CGA GGT TAG GAG GAAGAG G 3′ 5′ GAG GGT CGG CCT ATA AGA CC 3′ GT WMS114 5′ ACA AAC AGA AAATCA AAA CCC G 3′ 5′ ATC CAT CGC CAT TGG AGT G 3′ GA WMS115 5′ GAT GTTGCC ACT TGA GCA TG 3′ 5′ GAT TAG TCA AAT GGA ACA CCC C 3′ CA WMS119 5′TGA CTA ACA TCC TTT GTC ACG C 3′ 5′ CAT GTC TCA ACC ACC CAC AG 3′ GTimpWMS120 5′ GAT CCA CCT TCC TCT CTC TC 3′ 5′ GAT TAT ACT GGT GCC GAA AC 3′CT,CA WMS121 5′ TCC TCT ACA AAC AAA CAC AC 3′ 5′ CTC GCA ACT AGA GGT GTATG 3′ CA WMS122 5′ GGG TGG GAG AAA GGA GAT G 3′ 5′ AAA CCA TCC TCC ATCCTG G 3′ CT,CA WMS124 5′ GCC ATG GCT ATC ACC CAG 3′ 5′ ACT GTT CGG TGCAAT TTG AG 3′ CT,GTimp WMS126 5′ CAC ACG CTC CAC CAT GAC 3′ 5′ GTT GAGTTG ATG CGG GAG G 3′ CA WMS128 5′ AGC ACA TTT TAA CAC AGA TA 3′ 5′ ATCTGT GAA ATT TTG AAA AC 3′ CA WMS129 5′ TCA GTG GGC AAG CTA CAC AG 3′ 5′AAA ACT TAG TAG CCG CGT 3′ GTimp WMS130 5′ AGC TTT GCT TCA CGA GGA AG 3′5′ CTC CTC TTT ATA TCG CGT CCC 3′ GT WMS131 5′ AAT CCC CAC CGA TTC TTCTC 3′ 5′ AGT TCG TGG GTC TCT GAT GG 3′ CT WMS132 5′ TAC CAA ATC GAA ACACAT CAG G 3′ 5′ CAT ATC AAG GTC TCC TTC CCC 3′ GA,GAA WMS133 5′ ATC TAAACA AGA CGG CGG TG 3′ 5′ ATC TGT GAC AAC CGG TGA GA 3′ CT WMS134 5′ CATGGA ACT TAG ACA GAA TTG 3′ 5′ CAG TAC TTG GTA CTG AAC AGG 3′ CA WMS1355′ TGT CAA CAT CGT TTT GAA AAG G 3′ 5′ ACA CTG TCA ACC TGG CAA TG 3′ GAWMS136 5′ GAC AGC ACC TTG CCC TTT G 3′ 5′ CAT CGG CAA CAT GCT CAT C 3′CT WMS140 5′ ATG GAG ATA TTT GGC CTA CAA C 3′ 5′ CTT GAC TTC AAG GCG TGACA 3′ CT WMS144 5′ TTT GCT GTG GTA CGA AAC ATA C 3′ 5′ ACT CAC AAA TGTCTA ATA AAA C 3′ GT WMS146 5′ CCA AAA AAA CTG CCT GCA TG 3′ 5′ CTC TGGCAT TGC TCC TTG G 3′ GAimp WMS148 5′ GTG AGG CAG CAA GAG AGA AA 3′ 5′CAA AGC TTG ACT CAG ACC AAA 3′ CA WMS149 5′ CAT TGT TTT CTG CCT CTA GCC3′ 5′ CTA GCA TCG AAC CTG AAC AAG 3′ GA WMS153 5′ GAT CTC GTC ACC CGGAAT TC 3′ 5′ TGG TAG AGA AGG ACG GAG AG 3′ GA WMS154 5′ TCA CAG AGA GAGAGG GAG GG 3′ 5′ ATG TGT ACA TGT TGC CTG CA 3′ GA WMS155 5′ CAA TCA TTTCCC CCT CCC 3′ 5′ AAT CAT TGG AAA TCC ATA TGC C 3′ CT WMS156 5′ CCA ACCGTG CTA TTA GTC ATT C 3′ 5′ CAA TGC AGG CCC TCC TAA C 3′ GT WMS157 5′GTC GTC GCG GTA AGC TTG 3′ 5′ GAG TGA ACA CAC GAG GCT TG 3′ CT WMS159 5′GGG CCA ACA CTG GAA CAC 3′ 5′ GCA GAA GCT TGT TGG TAG GC 3′ GT WMS160 5′TTC AAT TCA GTC TTG GCT TGG 3′ 5′ CTG CAG GAA AAA AAG TAC ACC C 3′ GAWMS161 5′ GAT CGA GTG ATG GCA GAT GG 3′ 5′ TGT GAA TTA CTT GGA CGT GG 3′CT WMS162 5′ AGT GGA TCG ACA AGG CTC TG 3′ 5′ AGA AGA AGC AAA GCC TTC CC3′ CA WMS163 5′ ACC TCG ACA GAC CTG GTA CG 3′ 5′ GTC TTT GTC ACC CGA TGGAC 3′ CT WMS164 5′ ACA TTT CTC CCC CAT CGT C 3′ 5′ TTG TAA ACA AAT CGCATG CG 3′ CT WMS165 5′ TGC AGT GGT CAG ATG TTT CC 3′ 5′ CTT TTC TTT CAGATT GCG CC 3′ GA WMS169 5′ ACC ACT GCA GAG AAC ACA TAC G 3′ 5′ GTG CTCTGC TCT AAG TGT GGG 3′ GA WMS174 5′ GGG TTC CTA TCT GGT AAA TCC C 3′ 5′GAC ACA CAT GTT CCT GCC AC 3′ CT WMS179 5′ AAG TTG AGT TGA TGC GGG AG 3′5′ CCA TGA CCA GCA TCC ACT C 3′ GT WMS180 5′ ATC CGC CTA AGG AAT AGT GT3′ 5′ GAT CGC ACG GGA GAG AGT 3′ CT WMS181 5′ TCA TTG GTA ATG AGG AGA GA3′ 5′ GAA CCA TTC ATG TGC ATG TC 3′ GA WMS182 5′ TGA TGT AGT GAG CCC ATAGGC 3′ 5′ TTG CAC ACA GCC AAA TAA GG 3′ CT WMS186 5′ GCA GAG CCT GGT TCAAAA AG 3′ 5′ CGC CTC TAG CGA GAG CTA TG 5′ GA WMS189 5′ AGG AGC AGC GGAACG AAC 3′ 5′ AGA AAT ACG GAA ACC CAC CC 3′ CA WMS190 5′ GTG CTT GCT GAGCTA TGA GTC 3′ 5′ GTG CCA CGT GGT ACC TTT G 3′ CT,GT WMS191 5′ AGA CTGTTG TTT GCG GGC 3′ 5′ TAG CAC GAC AGT TGT ATG CAT G 3′ CT WMS192 5′ GGTTTT CTT TCA GAT TGC GC 3′ 5′ CGT TGT CTA ATC TTG CCT TGC 3′ CT WMS193 5′CTT TGT GCA CCT CTC TCT CC 3′ 5′ AAT TGT GTT GAT GAT TTG GGG 3′ CT,CAWMS194 5′ GAT CTG CTC TAC TCT CCT CC 3′ 5′ CGA CGC AGA ACT TAA ACA AG 3′CT WMS195 5′ AGG TGC CGT CGC GTC TAC 3′ 5′ ACC CCC CAC GTC AGA GAG 3′ CTWMS197 5′ GAG AAA GAG GTC TGG AGG TCG 3′ 5′ CAA AAT GCA CAA GAA TGG AGG3′ CT WMS198 5′ TTG AAC CGG AAG GAG TAC AG 3′ 5′ TCA GTT TAT TTT GGG CATGTG 3′ CA WMS200 5′ TCA AGG GAA CAG ATG AGC G 3′ 5′ GAC CTG ATG AGA GCAAGC AC 3′ CT WMS203 5′ CCC AAA GCA GCG CAA GC 3′ 5′ ACC AAT GCT ATC GGCTCG 3′ CA,GA WMS205 5′ CGA CCC GGT TCA CTT CAG 3′ 5′ AGT CGC CGT TGT ATAGTG CC 3′ CT WMS210 5′ TGC ATC AAG AAT AGT GTG GAA G 3′ 5′ TGA GAG GAAGGC TCA CAC CT 3′ GA WMS212 5′ AAG CAA CAT TTG CTG CAA TG 3′ 5′ TGC AGTTAA CTT GTT GAA AGG A 3′ CT WMS213 5′ TGC CTG GCT CGT TCT ATC TC 3′ 5′CTA GCT TAG CAC TGT CGC CC 3′ GA WMS218 5′ CGG CAA ACG GAT ATC GAC 3′ 5′AAC AGT AAC TCT CGC CAT AGC C 3′ CT WMS219 5′ GAT GAG CGA CAC CTA GCC TC3′ 5′ GGG GTC CGA GTC CAC AAC 3′ GAimp WMS224 5′ TGA GTC CAG CAC TGC TGC3′ 5′ CAA CAT CCG CTC GTA TTC AA 3′ CT WMS228 5′ TCA TAT GCA CCT CTT TCCTAG G 3′ 5′ GTG TGC CAC CTT TGA CGT C 3′ CT,CA WMS231 5′ AGC TCG GGA TGAAGC GTG 3′ 5′ GAT CCG CCG CTG CGT TT 3′ GAimp WMS232 5′ ATC TCA ACG GCAAGC CG 3′ 5′ CTG ATG CAA GCA ATC CAC C 3′ GA WMS233 5′ TCA AAA CAT AAATGT TCA TTG GA 3′ 5′ TCA ACC GTG TGT AAT TTT GTC C 3′ CT WMS234 5′ GAGTCC TGA TGT GAA GCT GTT G 3′ 5′ CTC ATT GGG GTG TGT ACG TG 3′ CT,CAWMS237 5′ GAA TCA CTT GTG AAG CAT CTG G 3′ 5′ CTG GAT GCA TCA CAT CCA AC3′ CT WMS238 5′ TCG CTT CTA CCG CTC ACC 3′ 5′ AGT GCC TTG CCG AGG TC 3′CT,GT,GGGT WMS241 5′ TCT TCC AAC TAA AGC ATA GC 3′ 5′ CTT CCA TGG ACTACA TAC TAG C 3′ GA WMS242 5′ TCC AAG GCA GTA GGC AGG 3′ 5′ TGT TGT TGGCCT GTA TGC AT 3′ GA WMS244 5′ GGC AGC TGA GGC AAT CTG 3′ 5′ TTT GGA CATTTC CCA GCG 3′ CAimp WMS245 5′ CAG CGC AGT TAG CTC GC 3′ 5′ ATC TGT CCATTC GAG CGC 3′ CT WMS247 5′ GCA ATC TTT TTT CTG ACC ACG 3′ 5′ ATG TGCATG TCG GAC GC 3′ GA WMS248 5′ AGG ACT TCC GCA CCC TG 3′ 5′ TGG CGT GGTCTA AAT GGA C 3′ CA WMS249 5′ CAA ATG GAT CGA GAA AGG GA 3′ 5′ CTG CCATTT TTC TGG ATC TAC C 3′ GAimp WMS251 5′ CAA CTG GTT GCT ACA CAA GCA 3′5′ GGG ATG TCT GTT CCA TCT TAG 3′ CA WMS252 5′ CAA CTG TAC GTA GGT TTCATT GC 3′ 5′ TCT GCC GTA AGT CGC CTC 3′ GA WMS257 5′ AGA GTG CAT GGT GGGACG 3 5′ CCA AGA CGA TGC TGA AGT CA 3′ GT WMS258 5′ GAT CGC TTC ATC TCTCTC TCT C 3′ 5′ GTA CAC GCC GTA GGC CC 3′ CT WMS259 5′ AGG GAA AAG ACATCT TTT TTT TC 3′ 5′ CGA CCG ACT TCG GGT TC 3′ GA WMS260 5′ GCC CCC TTGCAC AAA TC 3′ 5′ CGC AGC TAC AGG AGG CC 3′ GA WMS261 5′ CTC CCT GTA CCCCTA AGG C 3′ 5′ CTC GCG CTA CTA GCC ATT G 3′ CT WMS263 5′ TCT GCC GTAAGT CGC CTC 3′ 5′ GGT TTC ATT GCT TGC CCT AA 3′ CT WMS264 5′ GAG AAA CATGCC GAA CAA CA 3′ 5′ GCA TGC ATG AGA ATA GGA ACT G 3′ CA WMS265 5′ TGTTGC GGA TGG TCA CTA TT 3′ 5′ GAG TAC ACA TTT GGC CTC TGC 3′ GT WMS268 5′AGG GGA TAT GTT GTC ACT CCA 3′ 5′ TTA TGT GAT TGC GTA CGT ACC C 3′ GAimpWMS269 5′ TGC ATA TAA ACA GTC ACA CAC CC 3′ 5′ TTT GAG CTC CAA AGT GAGTTA GC 3′ CA WMS271 5′ CAA GAT CGT GGA GCC AGC 3′ 5′ AGC TGC TAG CTT TTGGGA CA 3′ CT,GA WMS272 5′ TGC TCT TTG GCG AAT ATA TGG 3′ 5′ GTT CAA AACAAA TTA AAA GGC CC 3′ CA WMS273 5′ ATT GGA CGG ACA GAT GCT TT 3′ 5′ AGCAGT GAG GAA GGG GAT C 3′ GA WMS274 5′ AAC TTG CAA AAC TGT TCT GA 3′ 5′TAT TTG AAG CGG TTT GAT TT 3′ GT WMS275 5′ AAT TTT CTT CCT CAC TTA TTC T3′ 5′ AAC AAA AAA TTA GGG CC 3′ CT WMS276 5′ ATT TGC CTG AAG AAA ATA TT3′ 5′ AAT TTC ACT GCA TAC ACA AG 3′ CT WMS278 5′ GTT GCT TCA TGA ACG CTCAA 3′ 5′ CTG CCC AAT TTT CTC CAC TC 3′ GTimpGAimp WMS281 5′ CGG CCA TATTTC TGT AAG TAT GC 3′ 5′ GCA GGT AAT GGC CGG AC 3′ GT WMS282 5′ TTG GCCGTG TAA GGC AG 3′ 5′ TCT CAT TCA CAC ACA ACA CTA GC 3′ GA WMS284 5′ AATGAA AAA ACA CTT GCG TGG 3′ 5′ GCA CAT TTT TCA CTT TCG GG 3′ GA WMS285 5′ATG ACC CTT CTG CCA AAC AC 3′ 5′ ATC GAC CGG GAT CTA GCC 3′ GA WMS291 5′CAT CCC TAC GCC ACT CTG C 3′ 5′ AAT GGT ATC TAT TCC GAC CCG 3′ CA WMS2925′ TCA CCG TGG TCA CCG AC 3′ 5′ CCA CCG AGC CGA TAA TGT AC 3′ CT WMS2935′ TAC TGG TTC ACA TTG GTG CG 3′ 5′ TCG CCA TCA CTC GTT CAA G 3′ CAWMS294 5′ GGA TTG GAG TTA AGA GAG AAC CG 3′ 5′ GCA GAG TGA TCA ATG CCAGA 3′ GAimp WMS295 5′ GTG AAG CAG ACC CAC AAC AC 3′ 5′ GAC GGC TGC GACGTA GAG 3′ GA WMS296 5′ AAT TCA ACC TAC CAA TCT CTG 3′ 5′ GCC TAA TAAACT GAA AAC GAG 3′ CT WMS297 5′ ATC GTC ACG TAT TTT GCA ATG 3′ 5′ TGCGTA AGT CTA GCA TTT TCT G 3′ GT,GA WMS299 5′ ACT ACT TAG GCC TCC CGC C3′ 5′ TGA CCC ACT TGC AAT TCA TC 3′ GA,TAG WMS301 5′ GAG GAG TAA GAC ACATGC CC 3′ 5′ GTG GCT GGA GAT TCA GGT TC 3 GA,G WMS302 5′ GCA AGA AGC AACAGC AGT AAC 3′ 5′ CAG ATG CTC TTC TCT GCT GG 3′ GA WMS304 5′ AGG AAA CAGAAA TAT CGC GG 3′ 5′ AGG ACT GTG GGG AAT GAA TG 3′ CT WMS311 5′ TCA CGTGGA AGA CGC TCC 3′ 5′ CTA CGT GCA CCA CCA TTT TG 3′ GA WMS312 5′ ATC GCATGA TGC ACG TAG AG 3′ 5′ ACA TGC ATG CCT ACC TAA TGG 3′ GA WMS313 5′ CCGCCC TCA TTA AGT TTC AC 3′ 5′ TTT GAC AAG TAC ACG ACT CTG C 3′ CT,GTWMS314 5′ AGG AGC TCC TCT GTG CCA C 3′ 5′ TTC GGG ACT CTC TTT CCC TG 3′CT WMS316 5′ CAT GGA CAT TTT ACC ACA AGA C 3′ 5′ TGC GTG TGG TCC ACC TC3′ AT,GT WMS319 5′ GGT TGC TGT ACA AGT GTT CAC G 3′ 5′ CGG GTG CTG TGTGTA ATG AC 3′ CT WMS320 5′ CGA GAT ACT ATG GAA GGT GAG G 3′ 5′ ATC TTTGCA AGG ATT GCC C 3′ GT,GA WMS321 5′ CAA TGT GGA GAG GGT GTG C 3′ 5′ TGTTGC ATG CGA TCA TGC 3′ GT,GAimp WMS322 5′ TCA CAA AAT GAT TTC TCA TCC G3′ 5′ TGC AGA AAA CCA ACA AGG G 3′ GA WMS325 5′ TTT CTT CTG TCG TTC TCTTCC C 3′ 5′ TTT TTA CGC GTC AAC GAC G 3′ CT WMS328 5′ GCA ATC CAC GAGAAG AGA GG 3′ 5′ CAC AAA CTC TTG ACA TGT GCG 3′ GT WMS330 5′ TTG CTA TCCATG TGC CAG AG 3′ 5′ ACA TGT TTC ATG CAG GTA GCC 3′ GTT WMS332 5′ AGCCAG CAA GTC ACC AAA AC 3′ 5′ AGT GCT GGA AAG AGT AGT GAA GC 3′ GA WMS3335′ GCC CGG TCA TGT AAA ACG 3′ 5′ TTT CAG TTT GCG TTA AGC TTT G 3′ GAWMS334 5′ AAT TTC AAA AAG GAG AGA GA 3′ 5′ AAC ATG TGT TTT TAG CTA TC 3′GA WMS335 5′ CGT ACT CCA CTC CAC ACG G 3′ 5′ CGG TCC AAG TGC TAC CTT TC3′ GA,GCGT WMS336 5′ CCC TTT AAT CTC GCT CCC TC 3′ 5′ GTC TCT TTC TCGTAC TTC CAG G 3′ CT WMS337 5′ CCT CTT CCT CCC TCA CTT AGC 3′ 5′ TGC TAACTG GCC TTT GCC 3′ CT,CACT,CA WMS339 5′ AAT TTT CTT CCT CAC TTA TT 3′ 5′AAA CGA ACA ACC ACT CAA TC 3′ CT WMS340 5′ GCA ATC TTT TTT CTG ACC ACG3′ 5′ ACG AGG CAA GAA CAC ACA TG 3′ GA WMS341 5′ TTC AGT GGT AGC GGT CGAG 3′ 5′ CCG ACA TCT CAT GGA TCC AC 3′ CT WMS342 5′ TAT CCA GAG CAG ACGGAC G 3′ 5′ GGT CTA GCT TCG ACG ACA CC 3′ GT WMS344 5′ CAA GGA AAT AGGCGG TAA CT 3′ 5′ ATT TGA GTC TGA AGT TTG CA 3′ GT WMS346 5′ CAA GCA AGGTTT CGT TTT ATC C 3′ 5′ GCA TGT GGT CCA TGT ACT GC 3′ AT,GT WMS349 5′GGC TTC CAG AAA ACA ACA GG 3′ 5′ ATC GGT GCG TAC CAT CCT AC 3′ GA WMS3505′ ACC TCA TCC ACA TGT TCT ACG 3′ 5′ GCA TGG ATA GGA CGC CC 3′ GT WMS3535′ CCA TGT TGA GTA GGT TCA GCC 3′ 5′ CTT GGC CAG AAG CTA CGA AC 3′GCGT,GT WMS356 5′ AGC GTT CTT GGG AAT TAG AGA 3′ 5′ CCA ATC AGC CTG CAACAA C 3′ GA WMS357 5′ TAT GGT CAA AGT TGG ACC TCG 3′ 5′ AGG CTG CAG CTCTTC TTC AG 3′ GA WMS358 5′ AAA CAG CGG ATT TCA TCG AG 3′ 5′ TCC GCT GTTGTT CTG ATC TC 3′ GAimp WMS359 5′ CTA ATT GCA ACA GGT CAT GGG 3′ 5′ TACTTG TGT TCT GGG ACA ATG G 3′ CT,CTTimp WMS361 5′ GTA ACT TGT TGC CAA AGGGG 3′ 5′ ACA AAG TGG CAA AAG GAG ACA 3′ GAimp WMS368 5′ CCA TTT CAC CTAATG CCT GC 3′ 5′ AAT AAA ACC ATG AGC TCA CTT GC 3′ AT WMS369 5′ CTG CAGGCC ATG ATG ATG 3′ 5′ ACC GTG GGT GTT GTG AGC 3′ CTimp WMS371 5′ GAC CAAGAT ATT CAA ACT GGC C 3′ 5′ AGC TCA GCT TGC TTG GTA CC 3′ CA,GA WMS3725′ AAT AGA GCC CTG GGA CTG GG 3′ 5′ GAA GGA CGA CAT TCC ACC TG 3′ GAWMS374 5′ ATA GTG TGT TGC ATG CTG TGT G 3′ 5′ TCT AAT TAG CGT TGG CTG CC3′ GT WMS375 5′ ATT GGC GAC TCT AGC ATA TAC G 3′ 5′ GGG ATG TCT GTT CCATCT TAG C 3′ CA WMS376 5′ GGG CTA GAA AAC AGG AAG GC 3′ 5′ TCT CCC GGAGGG TAG GAG 3′ CA,GAimp WMS382 5′ GTC AGA TAA CGC CGT CCA AT 3′ 5′ CTACGT GCA CCA CCA TTT TG 3′ GA WMS383 5′ ACG CCA GTT GAT CCG TAA AC 3′ 5′GAC ATC AAT AAC CGT GGA TGG 3′ GT WMS384 5′ TTT TCA TTG TGC CCT CTA CT3′ 5′ GCC AAG TTT CTT AGC TAG TTA A 3′ GTimp WMS388 5′ CTA CAA TTC GAAGGA GAG GGG 3′ 5′ CAC CGC GTC AAC TAC TTA AGC 3′ CT,CA,CA WMS389 5′ ATCATG TCG ATC TCC TTG ACG 3′ 5′ TGC CAT GCA CAT TAG CAG AT 3′ CT,GT WMS3905′ AAG TTT CAC ACA AGA TCT CTC C 3′ 5′ TGA CAA GTA CAC GAG TCT GC 3′CT,GT WMS391 5′ ATA GCG AAG TCT CCC TAC TCC A 3′ 5′ ATG TGC ATG TCG GACGC 3′ CA,GA WMS393 5′ TCA TCT GCT ATT TGT GCT ACA 3′ 5′ TCA AAT ACA CCAATG TGC C 3′ CA WMS395 5′ TAC AAC CGC AAG TAA TGC CA 3′ 5′ TAC CAA CACCCT AGC CCT TG 3′ CA WMS397 5′ TGT CAT GGA TTA TTT GGT CGG 3′ 5′ CTG CACTCT CGG TAT ACC AGC 3′ CT WMS400 5′ GTG CTG CCA CCA CTT GC 3′ 5′ TGT AGGCAC TGC TTG GGA G 3′ CA WMS403 5′ CGA CAT TGG CTT CGG TG 3′ 5′ ATA AAACAG TGC GGT CCA GG 3′ CA WMS408 5′ TCG ATT TAT TTG GGC CAC TG 3′ 5′ GTATAA TTC GTT CAC AGC ACG C 3′ CA WMS410 5′ GCT TGA GAC CGG CAC AGT 3′ 5′CGA GAC CTT GAG GGT CTA GA 3′ CA WMS411 5′ CCC ATA CGA TGA TGT GTT TCC3′ 5′ CAA ACG GAA CAT GGT CCC 3′ CT WMS412 5′ ATC AAC AAG GTT TGT GTGTTG G 3′ 5′ ATG AAA CGC GAC CTC CC 3′ GA WMS413 5′ TGC TTG TCT AGA TTGCTT GGG 3′ 5′ GAT CGT CTC GTC CTT GGC A 3′ GA WMS415 5′ GAT CTC CCA TGTCCG CC 3′ 5′ CGA CAG TCG TCA CTT GCC TA 3′ GAimp WMS425 5′ GAG CCC ACAAGC TGG CA 3′ 5′ TCG TTC TCC CAA GGC TTG 3′ CT WMS427 5′ AAA CTT AGA ACTGTA ATT TCA GA 3′ 5′ AGT GTG TTC ATT TGA CAG TT 3′ CA WMS428 5′ CGA GGCAGC GAG GAT TT 3′ 5′ TTC TCC ACT AGC CCC GC 3′ GA WMS429 5′ TTG TAC ATTAAG TTC CCA TTA 3′ 5′ TTT AAG GAC CTA CAT GAC AC 3′ CT WMS434 5′ ATG AGTTCC GCC AAA GAA TG 3′ 5′ ACG AAA TAC ACA AGT GGG ACA 3′ GT WMS437 5′ GATCAA GAC TTT TGT ATC TCT C 3′ 5′ GAT GTC CAA CAG TTA GCT TA 3′ CT WMS4405′ CCT ATG GTC TCC ATC ATG AGG 3′ 5′ TCA TGT CAA CTC AAG AAC ACG 3′ CTWMS443 5′ GGG TCT TCA TCC GGA ACT CT 3′ 5′ CCA TGA TTT ATA AAT TCC ACC3′ CA,GA WMS445 5′ TTT GTT GGG GGT TAG GAT TAG 3′ 5′ CCT TAA CAC TTG CTGGTA GTG A 3′ CT WMS448 5′ AAA CCA TAT TGG GAG GAA AGG 3′ 5′ CAC ATG GCATCA CAT TTG TG 3′ GA WMS455 5′ ATT CGG TTC GCT AGC TAC CA 3′ 5′ ACG GAGAGC AAC CTG CC 3′ GTimp WMS456 5′ TCT GAA CAT TAC ACA ACC CTG A 3′ 5′TGC TCT CTC TGA ACC TGA AGC 3′ GA WMS458 5′ AAT GGC AAT TGG AAG ACA TAGC 3′ 5′ TTC GCA ATG TTG ATT TGG C 3′ CA WMS459 5′ ATG GAG TGG TCA CACTTT GAA 3′ 5′ AGC TTC TCT GAC CAA CTT CTC G 3′ GA WMS469 5′ CAA CTC AGTGCT CAC ACA ACG 3′ 5′ CGA TAA CCA CTC ATC CAC ACC 3′ CT WMS471 5′ CGGCCC TAT CAT GGC TG 3′ 5′ GCT TGC AAG TTC CAT TTT GC 3′ CA WMS473 5′ TCATAC GGG TAT GGT TGG AC 3′ 5′ CAC CCC CTT GTT GGT CAC 3′ GTimp WMS476 5′ATG GGT TCG TAC TAA CAT CAG C 3′ 5′ TTG CTG GTA GCT TCA ATC CC 3′ GAimpWMS480 5′ TGC TGC TAC TTG TAC AGA GGA C 3′ 5′ CCG AAT TGT CCG CCA TAG 3′CT,CA WMS484 5′ ACA TCG CTC TTC ACA AAC CC 3′ 5′ AGT TCC GGT CAT GGC TAGG 3′ CT WMS494 5′ ATT GAA CAG GAA GAC ATC AGG G 3′ 5′ TTC CTG GAG CTGTCT GGC 3′ CA WMS495 5′ GAG AGC CTC GCG AAA TAT AGG 3′ 5′ TGC TTC TGGTGT TCC TTC G 3′ GA WMS497 5′ GTA GTG AAG ACA AGG GCA TT 3′ 5′ CCG AAAGTT GGG TGA TAT AC 3′ GTimp WMS499 5′ ACT TGT ATG CTC CAT TGA TTG G 3′5′ GGG GAG TGG AAA CTG CAT AA 3′ GA WMS501 5′ GGC TAT CTC TGG CGC TAA AA3′ 5′ TCC ACA AAC AAG TAG CGC C 3′ CA WMS512 5′ AGC CAC CAT CAG CAA AAATT 3′ 5′ GAA CAT GAG CAG TTT GGC AC 3′ GT WMS513 5′ ATC CGT AGC ACC TACTGG TCA 3′ 5′ GGT CTG TTC ATG CCA CAT TG 3′ CA WMS515 5′ AAC ACA ATG GCAAAT GCA GA 3′ 5′ CCT TCC TAG TAA GTG TGC CTC A 3′ GTimp WMS518 5′ AATCAC AAC AAG GCG TGA CA 3′ 5′ CAG GGT GGT GCA TGC AT 3′ CA WMS530 5′ AAATAG GAC AAC CCA CGG C 3′ 5′ TCA ACT TCT TGG CCT CCA TC 3′ CT WMS532 5′ACT GCG TGT GCC TAC AAT TG 3′ 5′ TCA CTC GCA CTC GAT AGG C 3′ GT WMS5335′ AAG GCG AAT CAA ACG GAA TA 3′ 5′ GTT GCT TTA GGG GAA AAG CC 3′ CT,CAWMS537 5′ ACA TAA TGC TTC CTG TGC ACC 3′ 5′ GCC ACT TTT GTG TCG TTC CT3′ CA,TA WMS538 5′ GCA TTT CGG GTG AAC CC 3′ 5′ GTT GCA TGT ATA CGT TAAGCG G 3′ GTimp WMS540 5′ TCT CGC TGT GAA ATC CTA TTT C 3′ 5′ AGG CAT GGATAG AGG GGC 3′ CTimp WMS544 5′ TAG AAT TCT TTA TGG GGT CTG C 3′ 5′ AGGATT CCA ATC CTT CAA AAT T 3′ CT,ATCT,CT WMS550 5′ CCC ACA AGA ACC TTTGAA GA 3′ 5′ CAT TGT GTG TGC AAG GCA C 3′ CT,GT WMS554 5′ TGC CCA CAACGG AAC TTG 3′ 5′ GCA ACC ACC AAG CAC AAA GT 3′ CT,GTimp WMS565 5′ GCGTCA GAT ATG CCT ACC TAG G 3 5′ AGT GAG TTA GCC CTG AGC CA 3′ CA WMS5665′ TCT GTC TAC CCA TGG GAT TTG 3′ 5′ CTG GCT TCG AGG TAA GCA AC 3′ CA,TAWMS569 5′ GGA AAC TTA TTG ATT GAA AT 3′ 5′ TCA ATT TTG ACA GAA GAA TT 3′GT WMS570 5′ TCG CCT TTT ACA GTC GGC 3′ 5′ ATG GGT AGC TGA GAG CCA AA 3′CT,GT WMS573 5′ AAG AGA TAA CAT GCA AGA AA 3′ 5′ TTC AAA TAT GTG GGA ACTAC 3′ CA WMS577 5′ ATG GCA TAA TTT GGT GAA ATT G 3′ 5′ TGT TTC AAG CCCAAC TTC TAT T 3′ CA,TA WMS582 5′ AAG CAC TAC GAA AAT ATG AC 3′ 5′ TCTTAA GGG GTG TTA TCA TA 3′ CA WMS583 5′ TTC ACA CCC AAC CAA TAG CA 3′ 5′TCT AGG CAG ACA CAT GCC TG 3′ CA WMS588 5′ GAT CCC CAA TTG CAT GTT G 3′5′ CTT GCA ACT GGG GGA CAC 3′ GT


6. A method for the preparation of a microsatellite marker of claims 1to 5 for plants of the Triticum aestivum species as well of the TribeTriticeae, characterized in that hypervariable genome sections(so-called microsatellites), with the help of the polymerase chainreaction (PCR), are amplified, subsequently separated and detected topolymorphous fragments in the presence of two specific primers, whichflank a microsatellite sequence to the left and right of eachmicrosatellite locus.
 7. The method of claim 6, characterized in thathighly resolving agarose gels, native polyacrylamide gels or denaturingpolyacrylamide gels are used for the separation of the markers.
 8. Themethod of claim 6, characterized in that, depending on the separationsystem, the detection is carried out by means of ethidium bromidestaining, silver staining, radiographic labeling followed byautoradiography or by means of automatic sequencing equipment using dye-or fluorescence-labeled primers.
 9. The use of the microsatellitemarkers of claims 1 to 7, for the genetic analysis of hexaploid andtetraploid cultivated forms of wheat.
 10. The use according to claim 8for the genetic mapping and marking of monogenic and polygenicproperties and their selection for analyzing relationships andidentifying varieties, as well as for evaluating the purity ofvarieties, identifying hybrids and breeding plants.